Journal Article

Group B Streptococcus GAPDH Is Released upon Cell Lysis, Associates with Bacterial Surface, and Induces Apoptosis in Murine Macrophages

Poyart, Claire, Oliveira, Liliana, Madureira, Pedro, Trieu-Cuot, Patrick, Andrade, Elva Bonifácio, Ferreira, Paula, Bouaboud, Abdelouhab, Morello, Eric, Dramsi, Shaynoor

2012

Overview

Glyceraldehyde 3-phosphate dehydrogenases (GAPDH) are cytoplasmic glycolytic enzymes that, despite lacking identifiable secretion signals, have been detected at the surface of several prokaryotic and eukaryotic organisms where they exhibit non-glycolytic functions including adhesion to host components. Group B Streptococcus (GBS) is a human commensal bacterium that has the capacity to cause life-threatening meningitis and septicemia in newborns. Electron microscopy and fluorescence-activated cell sorter (FACS) analysis demonstrated the surface localization of GAPDH in GBS. By addressing the question of GAPDH export to the cell surface of GBS strain NEM316 and isogenic mutant derivatives of our collection, we found that impaired GAPDH presence in the surface and supernatant of GBS was associated with a lower level of bacterial lysis. We also found that following GBS lysis, GAPDH can associate to the surface of many living bacteria. Finally, we provide evidence for a novel function of the secreted GAPDH as an inducer of apoptosis of murine macrophages.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Analysis / Animals / Antigens, Surface / Antigens, Surface - genetics / Antigens, Surface - metabolism / Antigens, Surface - physiology / Apoptosis / Apoptosis - drug effects / Apoptosis - physiology / B cells / Bacteria / Bacterial Adhesion / Bacterial Adhesion - physiology / Bacterial Proteins / Bacterial Proteins - analysis / Bacterial Proteins - metabolism / Bacterial Proteins - pharmacology / Bacterial Proteins - physiology / Bacteriology / Biology / Cell Extracts / Cell Extracts - chemistry / Cell Extracts - secretion / Cell surface / Cells, Cultured / Dehydrogenases / Electron microscopy / Enzymes / Female / Fimbriae, Bacterial / Fimbriae, Bacterial - genetics / Fimbriae, Bacterial - metabolism / Fimbriae, Bacterial - physiology / Flow cytometry / Fluorescence / Gangrene / Glyceraldehyde / Glyceraldehyde 3-phosphate / Glyceraldehyde-3-phosphate dehydrogenase / Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - analysis / Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - metabolism / Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - pharmacology / Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - physiology / Glycolysis / Laboratories / Life Sciences / Listeria / Listeria monocytogenes / Localization / Lysis / Macrophages / Macrophages - drug effects / Macrophages - pathology / Macrophages - physiology / Medicine / Meningitis / Mice / Mice, Inbred C57BL / Microbiology and Parasitology / Neonates / Organisms, Genetically Modified / Pathogens / Phosphates / Phosphorylation / Protein Binding / Proteins / Secretion / Secretion signals / Sepsis / Septicemia / Signal transduction / Streptococcaceae / Streptococcaceae - classification / Streptococcaceae - enzymology / Streptococcaceae - growth & development / Streptococcal Infections / Streptococcal Infections - immunology / Streptococcal Infections - metabolism / Streptococcal Infections - pathology / Streptococcus / Streptococcus infections