Journal Article

CD69 expression on regulatory T cells protects from immune damage after myocardial infarction

Robson, Simon C., Martín, Pilar, Blanco-Domínguez, Rafael, García-Guimaraes, Marcos M., Rivero, Fernando, Rodríguez, Cristina, Cuesta, Javier, Duran-Cambra, Albert, Taurón, Manel, Curtabbi, Andrea, Rodríguez-Arabaolaza, Iker, Alfonso, Fernando, Jiménez-Borreguero, Luis J., Enríquez, Jose Antonio, de la Fuente, Hortensia, Martínez-González, José, Vera, Alberto, Martín-Aguado, Laura, Villalba-Orero, María, Alonso, Judith, Cecconi, Alberto, Sánchez-Madrid, Francisco, Sánchez-Díaz, Raquel, Bueno, Héctor, Jiménez-Alejandre, Rosa

2022

Overview

Increasing evidence has pointed to the important function of T cells in controlling immune homeostasis and pathogenesis after myocardial infarction (MI), although the underlying molecular mechanisms remain elusive. In this study, a broad analysis of immune markers in 283 patients revealed significant CD69 overexpression on Tregs after MI. Our results in mice showed that CD69 expression on Tregs increased survival after left anterior descending (LAD) coronary artery ligation. Cd69-/- mice developed strong IL-17+ γδT cell responses after ischemia that increased myocardial inflammation and, consequently, worsened cardiac function. CD69+ Tregs, by induction of AhR-dependent CD39 ectonucleotidase activity, induced apoptosis and decreased IL-17A production in γδT cells. Adoptive transfer of CD69+ Tregs into Cd69-/- mice after LAD ligation reduced IL-17+ γδT cell recruitment, thus increasing survival. Consistently, clinical data from 2 independent cohorts of patients indicated that increased CD69 expression in peripheral blood cells after acute MI was associated with a lower risk of rehospitalization for heart failure (HF) after 2.5 years of follow-up. This result remained significant after adjustment for age, sex, and traditional cardiac damage biomarkers. Our data highlight CD69 expression on Tregs as a potential prognostic factor and a therapeutic option to prevent HF after MI.

• Publisher: American Society for Clinical Investigation
• Subject: Adoptive transfer / Adoptive Transfer - methods / Animals / Apoptosis / Atherosclerosis / Biomarkers / Biomedical research / Blood cells / Cardiology / Cardiovascular disease / CD69 antigen / Cell survival / Complications and side effects / Congestive heart failure / Coronary artery / Coronary vessels / Ectonucleotidase / Ejection fraction / Genetic aspects / Health aspects / Heart attack / Heart attacks / Heart failure / Heart Failure - genetics / Heart Failure - metabolism / Homeostasis / Immune response / Immunology / Immunoregulation / Inflammation / Interleukin 17 / Interleukin-17 - metabolism / Ischemia / Kinases / Lectins / Lymphocytes / Lymphocytes T / Medical prognosis / Mice / Molecular modelling / Myocardial infarction / Myocardial Infarction - pathology / Patients / Peripheral blood / Physiological aspects / Prevention / Prognosis / Risk factors / Suppressor cells / T-Lymphocytes, Regulatory