Journal Article

Determining host factors contributing to the reactivation of JC virus in kidney transplant recipients

Keykhosravi, Sajedeh, Ashrafkhani, Babak, Monfared, Ali, Hasan-alizadeh, Elham, Khosravi, Masoud, Hasandokht, Tolou, Shenagari, Mohammad, Noori Goodarzi, Narjes, Mosadegh, Mehrdad

2022

Overview

Background and aims The John Cunningham virus (JCV) is the established etiological agent of the polyomavirus-associated nephropathy among renal transplant recipients. In the present study, we aimed to determine the probable predictive factors leading to JCV replication in renal transplant patients. Material and methods Urine and plasma samples were collected from a total of 120 consecutive renal‐transplanted patients without preliminary screening from Jan 2018 to Mar 2019. After DNA extraction, the simultaneous detection and quantification of JCV and BK polyomavirus (BKV) were conducted using a Real-time quantitative PCR method. Moreover, statistical analyses were performed using the statistical software packages, SPSS version 21. Results The prevalence of JCV viruria and viremia among renal transplant recipients were 26 (21.67%) and 20 (16.67%), respectively. A significant association was observed between the JCV and two risk factors, diabetes mellitus ( P  = 0.002) and renal stones ( P  = 0.015). The prevalence of JCV viremia among recipients who were grafted near time to sampling was significantly higher ( P  = 0.02). There was a statistically significant coexistence between BK and JC viruses among our patients ( P  = 0.029). The frequency of JCV viruria in males was reported almost three times more than in females ( P  = 0.005). The JCV shedding in urine was significantly associated with the tropical steroids like prednisolone acetate, which have been the standard regimen ( P  = 0.039). Multivariable analysis revealed duration of post-transplantation (OR, 0.89; P  = 0.038), diabetes mellitus (OR, 1.85; P  = 0.034), and renal stone (OR 1.10; P  = 0.04) as independent risk factors associated with JCV viremia post-renal transplantation. Conclusion It seems that the discovery of potential risk factors, including immunological and non-immunological elements, may offer a possible preventive or therapeutic approach in the JCV disease episodes. The results of this study may also help clarify the probable clinical risk factors involving in progressive multifocal leukoencephalopathy development.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: acetates / Acetic acid / Biomedical and Life Sciences / Biomedicine / BK Virus / Calculi / Chi-square test / Clinical Virology / Coexistence / computer software / Diabetes / Diabetes mellitus / DNA / DNA virus infections / DNA, Viral - genetics / etiological agents / Female / Genetic aspects / Graft rejection / Host-virus relationships / Human polyomavirus 1 / Human polyomavirus 2 / Humans / Infections / JC virus / JC Virus - genetics / Kidney Diseases - virology / kidney transplant / Kidney transplantation / Kidney Transplantation - adverse effects / Kidney transplants / Kidneys / Leukoencephalopathy / Lithiasis / Male / Nephrolithiasis / Nephropathy / Patients / Physiological aspects / Plasma / Polyoma virus / Polyomavirus Infections / Prednisolone / Progressive multifocal leukoencephalopathy / quantitative polymerase chain reaction / Reactivation / renal calculi / Renal transplantation / risk / Risk factors / Statistical analysis / Statistics / Steroid hormones / Transplant Recipients / Transplantation / Tumor Virus Infections / Urine / Viral infections / Viremia / Viremia - epidemiology / Virology / Viruria / Virus activation / Virus research / Viruses