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Restoration of Brain Acid Soluble Protein 1 Inhibits Proliferation and Migration of Thyroid Cancer Cells
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Restoration of Brain Acid Soluble Protein 1 Inhibits Proliferation and Migration of Thyroid Cancer Cells
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Journal Article
Restoration of Brain Acid Soluble Protein 1 Inhibits Proliferation and Migration of Thyroid Cancer Cells
2016
Overview
Background： Brain acid soluble protein 1 （BASPI） is identified as a novel potential tumor suppressor in several cancers. However, its role in thyroid cancer has not been investigated yet. In the present study, the antitumor activities of BASP1 against the growth and migration of thyroid cancer cells were evaluated. Methods： BASP1 expression in thyroid cancer tissues and normal tissues were examined by immunohistochemical staining and the association between its expression and prognosis was analyzed, pcDNA-BASP 1 carrying full length of BASP1 cDNA was constructed to restore the expression of BASP1 in thyroid cancer cell lines （BHT- I 01 and KMH-2）. The cell proliferation in vitro and in vivo was evaluated by WST-1 assay and xenografl tumor models, respectively. Cell cycle distribution after transfection was analyzed using flow cytometry. Cell apoptosis after transfection was examined by annexin V/propidium iodide assay. The migration was examined using transwell assay. Results： BASP1 expression was abundant in normal tissues while it is significantly decreased in cancer tissues （P = 0.000）. pcDNA-BASP1 restored the expression of BASPI and significantly inhibited the growth of BHT-101 and KMH-2 cells as well as xenograft tumors in nude mice （P = 0.000）. pcDNA-BASPI induced G1 arrest and apoptosis in BHT-101 and KMH-2 cells. In addition, pcDNA-BASP1 significantly inhibited the cell migration. Conclusions： Downregulation of BASP1 expression may play a role in the tumorigenesis of thyroid cancer. Restoration of BASPI expression exerted extensive antitumor activities against growth and migration of thyroid cancer cells, which suggested that BASPI gene might act as a potential therapeutic agent for the treatment of thyroid cancer.
Publisher
Wolters Kluwer - Medknow Publications,Medknow Publications and Media Pvt. Ltd,Lippincott Williams & Wilkins Ovid Technologies,Department of General Surgery, Jiading Central Hospital, Shanghai 201822, China%Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China%Department of Pathology, Jiading Central Hospital, Shanghai 201822, China%Department of General Surgery, Jiading Central Hospital, Shanghai 201822, China%Department of Otorhinolaryngology, Zhongshan Hospital, Fudan University, Shanghai 200032, China%Department of General Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 201705, China,Department of General Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 201705, China,Medknow Publications & Media Pvt Ltd,Wolters Kluwer
Subject
/ Animals
/ Annexin
/ Brain Acid Soluble Protein 1; Gene Transfection; Proliferation; Thyroid Cancer
/ Calmodulin-Binding Proteins - genetics
/ Calmodulin-Binding Proteins - metabolism
/ Cell Proliferation - genetics
/ Cell Proliferation - physiology
/ Cytoskeletal Proteins - genetics
/ Cytoskeletal Proteins - metabolism
/ DNA
/ Female
/ Gene Expression Regulation, Neoplastic - genetics
/ Gene Expression Regulation, Neoplastic - physiology
/ Humans
/ Male
/ Melanoma
/ Membrane Proteins - genetics
/ Membrane Proteins - metabolism
/ Mice
/ Nerve Tissue Proteins - genetics
/ Nerve Tissue Proteins - metabolism
/ Original
/ Plasmids
/ Prostate
/ Proteins
/ Repressor Proteins - genetics
/ Repressor Proteins - metabolism
/ Studies
/ Surgery
/ Thyroid Neoplasms - metabolism
/ Thyroid Neoplasms - pathology
/ Tumors
/ Xenograft Model Antitumor Assays
/ 抗肿瘤活性
/ 甲状腺癌
/ 癌细胞
/ 细胞增殖
/ 细胞迁移
/ 蛋白
/ 酸溶性
