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Antimicrobial activity of D-amino acid in combination with photo-sonoactivated hypericin nanoparticles against Acinetobacter baumannii
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Antimicrobial activity of D-amino acid in combination with photo-sonoactivated hypericin nanoparticles against Acinetobacter baumannii
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Journal Article
Antimicrobial activity of D-amino acid in combination with photo-sonoactivated hypericin nanoparticles against Acinetobacter baumannii
2023
Overview
Background
The emergence of multidrug-resistant
Acinetobacter baumannii
strains is increasing worldwide. To overcome these life-threatening infections, the development of new treatment approaches is critical. For this purpose, this study was conducted to determine the antimicrobial photo-sonodynamic therapy (aPSDT) using hypericin nanoparticles (HypNP) in combination with D-Tryptophan (D-Trp) against
A. baumannii
.
Materials and methods
HypNP was synthesized and characterized, followed by the determination of the fractional inhibitory concentration (FIC) index of HypNP and D-Trp by checkerboard assay. Next, the antimicrobial and anti-biofilm potential of HypNP@D-Trp-mediated aPSDT against
A. baumannii
was evaluated. Finally, the anti-virulence activity of aPSDT using HypNP@D-Trp was accessed following the characterization of HypNP@D-Trp interaction with AbaI using in silico virtual screening and molecular docking.
Results
A synergistic activity in the combination of HypNP and D-Trp against
A. baumannii
was observed with a FIC index value of 0.5. There was a 5.10 log
10
CFU/mL reduction in the cell viability of
A. baumannii
when the bacterial cells were treated with 1/2 × MIC of HypNP@D-Trp and subsequently exposed to ultrasound waves and blue light (
P
< 0.05). Moreover, a significant biofilm degradation effect on biofilm-associated cells of
A. baumannii
was observed after treatment with aPSDT using 2 × MIC of HypNP@D-Trp in comparison with the control groups (
P
< 0.05). According to the molecular docking analysis of the protein-ligand complex, Hyp with a high affinity for AbaI showed a binding affinity of − 9.41 kcal/mol. Also, the expression level of
abaI
gene was significantly downregulated by 10.32-fold in
A. baumannii
treated with aPSDT as comprised with the control group (
P
< 0.05).
Conclusions
It can be concluded that HypNP@D-Trp-mediated aPSDT can be considered a promising strategy to overcome the infections caused by
A. baumannii
by reducing the growth of bacterial biofilm and decreasing the expression of
abaI
as a gene involved in
A. baumannii
biofilm formation.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Affinity
/ Anti-Bacterial Agents - pharmacology
/ Anti-Infective Agents - pharmacology
/ Antiinfectives and antibacterials
/ Antimicrobial photodynamic therapy
/ Antimicrobial sonodynamic therapy
/ Bacteria
/ Biofilms
/ Biomedical and Life Sciences
/ Control
/ Drug Resistance, Multiple, Bacterial
/ LDCs
/ Light
/ Minimum inhibitory concentration
/ Molecular Docking Simulation
/ Mycology
/ Peptides
/ Testing
/ Virology
