Asset Details
Do you wish to reserve the book?
Transcriptome analysis reveals mechanism of early ripening in Kyoho grape with hydrogen peroxide treatment
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Transcriptome analysis reveals mechanism of early ripening in Kyoho grape with hydrogen peroxide treatment
Do you wish to request the book?
Transcriptome analysis reveals mechanism of early ripening in Kyoho grape with hydrogen peroxide treatment
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Transcriptome analysis reveals mechanism of early ripening in Kyoho grape with hydrogen peroxide treatment
2020
Overview
Background
In a previous study, the early ripening of Kyoho grape following H
2
O
2
treatment was explored at the physiological level, but the mechanism by which H
2
O
2
promotes ripening at the molecular level is unclear. To reveal the molecular mechanism, RNA-sequencing analysis was conducted on the different developmental stages of Kyoho berry treated with H
2
O
2
.
Results
In the comparison of treatment and control groups, 406 genes were up-regulated and 683 were down-regulated. Time course sequencing (TCseq) analysis showed that the expression patterns of most of the genes were similar between the treatment and control, except for some genes related to chlorophyll binding and photosynthesis. Differential expression analysis and the weighted gene co-expression network were used to screen significantly differentially expressed genes and hub genes associated with oxidative stress (
heat shock protein
,
HSP
), cell wall deacetylation (
GDSL esterase/lipase
,
GDSL
), cell wall degradation (
xyloglucan endotransglucosylase/ hydrolase, XTH
), and photosynthesis (
chlorophyll a-b binding protein, CAB1
). Gene expression was verified with RT-qPCR, and the results were largely consistent with those of RNA sequencing.
Conclusions
The RNA-sequencing analysis indicated that H
2
O
2
treatment promoted the early ripening of Kyoho berry by affecting the expression levels of
HSP, GDSL, XTH,
and
CAB1
and- photosynthesis- pathways.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
