Journal Article

Timing of complementary feeding is associated with gut microbiota diversity and composition and short chain fatty acid concentrations over the first year of life

Benjamin-Neelon, Sara E., Hoyo, Cathrine, Mueller, Noel T., Differding, Moira K., Østbye, Truls

2020

Overview

Background Early introduction of complementary foods has been associated with various immune disorders, oxidative stress, and obesity in childhood. The gut microbiota and the short chain fatty acids (SCFAs) they produce are postulated to be on the causal pathway. The objective of this study was to determine if early complementary feeding (i.e. consumption of solids or non-water/formula liquids at or before 3 months) is prospectively associated with infant gut microbiota composition, diversity and SCFAs at 3 and 12 months of age in the Nurture birth cohort. Results Mother-infant dyads in the early complementary feeding group ( n  = 18) had similar baseline characteristics to those in the later feeding group ( n  = 49). We assessed differential abundance of microbial taxa (measured by 16S rRNA gene sequencing of the V4 region) by timing of complementary feeding using beta-binomial regression models (considering a two-sided FDR corrected p -value of < 0.05 as significant), and we fittted linear regression models to assess the association between early complementary feeding and SCFA concentrations (quantified using gas chromatography). After multivariable adjustment for breastfeeding, delivery method, birth weight, and gestational age, there were 13 differentially abundant microbial amplicon sequence variants (ASVs) by timing of introduction to complementary foods at 3 months and 20 ASVs at 12 months. Infants introduced to complementary foods early (vs. later) had higher concentrations of the SCFA butyric acid (mean difference = 0.65, 95% CI: 0.27, 1.04, p  < 0.01) and total SCFAs (mean difference = 38.8, 95% CI: 7.83, 69.7) at 12 months. Bilophila wadsworthia and Lachnospiraceae Roseburia were associated with early (vs. later) complementary feeding and with higher butyric acid concentrations at 3 and 12 months, respectively. Conclusions Our findings are consistent with the hypothesis that early (vs. later) introduction to complementary foods is associated with altered gut microbiota composition and butyric acid concentrations measured in stool until at least 1 year of age. Further research is needed to determine if these changes mediate future development of metabolic and immune conditions.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Age / Babies / Baby foods / Bacteria - classification / Bacteria - genetics / Bacteria - isolation & purification / Biodiversity / Biological Microscopy / Biomedical and Life Sciences / Breast feeding / Breastfeeding & lactation / Butyrate / Complementary feeding / DNA, Bacterial - genetics / DNA, Ribosomal - genetics / Fatty acids / Fatty Acids, Volatile - analysis / Feces / Feces - chemistry / Feces - microbiology / Female / Food / Gas chromatography / Gastrointestinal Microbiome / Genetic aspects / Gut microbiota / Humans / Infant / Infant nutrition / Infant Nutritional Physiological Phenomena / Infants / Intestinal microflora / Life Sciences / Male / Metabolites / Methods / Microbe-host interactions and microbial pathogenicity / Microbiology / Microbiome / Microbiota / Microbiota (Symbiotic organisms) / Microorganisms / Mothers / Mycology / Obesity / Observations / Oxidative stress / Parasitology / Pathogenicity / Pediatrics / Phylogeny / Physical growth / Physiological aspects / Prospective Studies / Research Article / RNA, Ribosomal, 16S - genetics / Sequence Analysis, DNA - methods / Studies / Time Factors / Virology / Weaning