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Journal Article
Imaging single glycans
2020
Overview
Imaging of biomolecules guides our understanding of their diverse structures and functions
1
,
2
. Real-space imaging at sub-nanometre resolution using cryo-electron microscopy has provided key insights into proteins and their assemblies
3
,
4
. Direct molecular imaging of glycans—the predominant biopolymers on Earth, with a plethora of structural and biological functions
5
—has not been possible so far
6
. The inherent glycan complexity and backbone flexibility require single-molecule approaches for real-space imaging. At present, glycan characterization often relies on a combination of mass spectrometry and nuclear magnetic resonance imaging to provide insights into size, sequence, branching and connectivity, and therefore requires structure reconstruction from indirect information
7
–
9
. Here we show direct imaging of single glycan molecules that are isolated by mass-selective, soft-landing electrospray ion beam deposition and imaged by low-temperature scanning tunnelling microscopy
10
. The sub-nanometre resolution of the technique enables the visualization of glycan connectivity and discrimination between regioisomers. Direct glycan imaging is an important step towards a better understanding of the structure of carbohydrates.
An imaging method combining soft-landing electrospray ion beam deposition and low-temperature scanning tunnelling microscopy resolves the structures of glycans at sub-nanometre resolution, revealing the connectivity of glycan chains and the types of linkages.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
