Journal Article

Rapid leukocyte migration by integrin-independent flowing and squeezing

Lämmermann, Tim, Wedlich-Söldner, Roland, Fässler, Reinhard, Worbs, Tim, Critchley, David R., Förster, Reinhold, Bader, Bernhard L., Monkley, Susan J., Sixt, Michael, Keller, Markus, Hirsch, Karin

2008

Overview

All metazoan cells carry transmembrane receptors of the integrin family, which couple the contractile force of the actomyosin cytoskeleton to the extracellular environment. In agreement with this principle, rapidly migrating leukocytes use integrin-mediated adhesion when moving over two-dimensional surfaces. As migration on two-dimensional substrates naturally overemphasizes the role of adhesion, the contribution of integrins during three-dimensional movement of leukocytes within tissues has remained controversial. We studied the interplay between adhesive, contractile and protrusive forces during interstitial leukocyte chemotaxis in vivo and in vitro . We ablated all integrin heterodimers from murine leukocytes, and show here that functional integrins do not contribute to migration in three-dimensional environments. Instead, these cells migrate by the sole force of actin-network expansion, which promotes protrusive flowing of the leading edge. Myosin II-dependent contraction is only required on passage through narrow gaps, where a squeezing contraction of the trailing edge propels the rigid nucleus. An extra dimension Leukocytes are remarkable in their ability to infiltrate any type of tissue almost anywhere in the body. On flat surfaces they migrate using transmembrane receptors of the integrin family — present in all metazoan cells — that couple the contractile force of the actomyosin cytoskeleton to the extracellular environment. But studies of cell migration on two-dimensional surfaces overemphasize the role of adhesion. The rapid movement and extreme flexibility of leukocytes in three dimensions is now shown — by genetic knockout — not to involve integrins. Instead, the cells migrate using an amoeba-like flowing and squeezing action, powered by actin network expansion alone. Leukocyte migration over two-dimensional surfaces is dependent on the integrin family of adhesion receptors, which couple the contractile force of the actomyosin cytoskeleton to the extracellular environment. In this study, all integrin heterodimers from mouse leukocytes were ablated and it is shown that integrins are not required for migration in 3D environments, in vitro and in vivo . Such non-adhesive migration renders leukocytes autonomous from the tissue environment.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: Actins - metabolism / Animals / Cell Adhesion / Cell adhesion & migration / Cell Movement / Cell Nucleus - metabolism / Cell Shape / Chemotaxis / Dendritic Cells - cytology / Dendritic Cells - metabolism / Genetics / Humanities and Social Sciences / Integrins / Integrins - deficiency / Integrins - genetics / Integrins - metabolism / Leukocytes / Leukocytes - cytology / Leukocytes - metabolism / Lymph Nodes - cytology / Lymph Nodes - immunology / Metazoa / Mice / multidisciplinary / Muscle proteins / Myosin / Myosin Type II - metabolism / Proteins / Science / Science (multidisciplinary) / Studies / Time Factors / Tissues