Asset Details
Do you wish to reserve the book?
Mycobacterium abscessus virulence traits unraveled by transcriptomic profiling in amoeba and macrophages
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Mycobacterium abscessus virulence traits unraveled by transcriptomic profiling in amoeba and macrophages
Do you wish to request the book?
Mycobacterium abscessus virulence traits unraveled by transcriptomic profiling in amoeba and macrophages
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Mycobacterium abscessus virulence traits unraveled by transcriptomic profiling in amoeba and macrophages
2019
Overview
Free-living amoebae are thought to represent an environmental niche in which amoeba-resistant bacteria may evolve towards pathogenicity. To get more insights into factors playing a role for adaptation to intracellular life, we characterized the transcriptomic activities of the emerging pathogen Mycobacterium abscessus in amoeba and murine macrophages (Mϕ) and compared them with the intra-amoebal transcriptome of the closely related, but less pathogenic Mycobacterium chelonae. Data on up-regulated genes in amoeba point to proteins that allow M. abscessus to resist environmental stress and induce defense mechanisms, as well as showing a switch from carbohydrate carbon sources to fatty acid metabolism. For eleven of the most upregulated genes in amoeba and/or Mϕ, we generated individual gene knock-out M. abscessus mutant strains, from which ten were found to be attenuated in amoeba and/or Mϕ in subsequence virulence analyses. Moreover, transfer of two of these genes into the genome of M. chelonae increased the intra-Mϕ survival of the recombinant strain. One knock-out mutant that had the gene encoding Eis N-acetyl transferase protein (MAB_4532c) deleted, was particularly strongly attenuated in Mϕ. Taken together, M. abscessus intra-amoeba and intra-Mϕ transcriptomes revealed the capacity of M. abscessus to adapt to an intracellular lifestyle, with amoeba largely contributing to the enhancement of M. abscessus intra-Mϕ survival.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Amoeba - growth & development
/ Animals
/ Bacteria
/ Bacterial Proteins - genetics
/ BCG
/ Biochemistry, Molecular Biology
/ Genes
/ Genomes
/ Genomics
/ Medicine and Health Sciences
/ Mice
/ Microbiology and Parasitology
/ Mycobacterium abscessus - genetics
/ Mycobacterium abscessus - isolation & purification
/ Mycobacterium abscessus - pathogenicity
/ Mycobacterium Infections, Nontuberculous - genetics
/ Mycobacterium Infections, Nontuberculous - microbiology
/ Proteins
/ Software
/ Survival
