Journal Article

Comparison of oral microbiota in tumor and non-tumor tissues of patients with oral squamous cell carcinoma

Li, Yihong, Estilo, Cherry, Pushalkar, Smruti, Saxena, Deepak, Yegnanarayana, Ramanathan, Ji, Xiaojie, Singh, Bhuvanesh, Li, Xin

2012

Overview

Background Bacterial infections have been linked to malignancies due to their ability to induce chronic inflammation. We investigated the association of oral bacteria in oral squamous cell carcinoma (OSCC/tumor) tissues and compared with adjacent non-tumor mucosa sampled 5 cm distant from the same patient ( n = 10 ). By using culture-independent 16S rRNA approaches, denaturing gradient gel electrophoresis (DGGE) and cloning and sequencing, we assessed the total bacterial diversity in these clinical samples. Results DGGE fingerprints showed variations in the band intensity profiles within non-tumor and tumor tissues of the same patient and among the two groups. The clonal analysis indicated that from a total of 1200 sequences characterized, 80 bacterial species/phylotypes were detected representing six phyla, Firmicutes , Bacteroidetes , Proteobacteria , Fusobacteria , Actinobacteria and uncultivated TM7 in non-tumor and tumor libraries. In combined library, 12 classes, 16 order, 26 families and 40 genera were observed. Bacterial species, Streptococcus sp . oral taxon 058, Peptostreptococcus stomatis , Streptococcus salivarius , Streptococcus gordonii , Gemella haemolysans, Gemella morbillorum , Johnsonella ignava and Streptococcus parasanguinis I were highly associated with tumor site where as Granulicatella adiacens was prevalent at non-tumor site. Streptococcus intermedius was present in 70% of both non-tumor and tumor sites. Conclusions The underlying changes in the bacterial diversity in the oral mucosal tissues from non-tumor and tumor sites of OSCC subjects indicated a shift in bacterial colonization. These most prevalent or unique bacterial species/phylotypes present in tumor tissues may be associated with OSCC and needs to be further investigated with a larger sample size.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Actinobacteria / Bacteria / Bacteria - classification / Bacteria - isolation & purification / Bacterial diseases / Bacterial infections / Biodiversity / Biological Microscopy / Biomedical and Life Sciences / Carcinoma, Squamous Cell - microbiology / Carcinoma, Squamous Cell - pathology / Cell cycle / Chronic infection / Cloning / Cloning, Molecular / Colon / Colonization / Colorectal cancer / Comparative analysis / Complications and side effects / Cytokines / Denaturing Gradient Gel Electrophoresis / Diseases / Female / Firmicutes / Gel electrophoresis / Gemella morbillorum / Genetic aspects / Granulicatella adiacens / Health aspects / Humans / Infections / Inflammation / Life Sciences / Male / Malignancy / Metagenome / Microbial diversity / Microbiology / Microbiota / Microbiota (Symbiotic organisms) / Microorganisms / Middle Aged / Mouth Mucosa - microbiology / Mouth Neoplasms - microbiology / Mouth Neoplasms - pathology / Mucosa / Mycology / Non-tumor / Oral cancer / Oral cavity / Oral squamous cell carcinoma / Parasitology / Pathogenesis / Peptostreptococcus / Physiological aspects / Proteobacteria / Research Article / RNA / RNA, Ribosomal, 16S - genetics / rRNA 16S / Sample size / Sequence Analysis, DNA / Squamous cell carcinoma / Streptococcus gordonii / Streptococcus intermedius / Streptococcus parasanguinis / Streptococcus salivarius / Studies / Tissues / Tumor / Tumors / United States / Viral infections / Virology