Journal Article

Novel plant flavonoid electrochemical sensor based on in-situ and controllable double-layered membranes modified electrode

Hu, Jing, Yang, Xin, Zhou, Renjie, Lin, Hongwei, Hu, Feilong, Wei, Qiuyuan

2020

Overview

Identification and quantification of plant flavonoids are critical to pharmacokinetic study and pharmaceutical quality control due to their distinct pharmacological functions. Here we report on a novel plant flavonoid electrochemical sensor for sensitive and selective detection of dihydromyricetin (DMY) based on double- layered membranes consisting of gold nanoparticles (Au) anchored on reduced graphene oxide (rGO) and molecularly imprinted polymers (MIPs) modified glassy carbon electrode (GCE). Both rGO-Au and MIPs membranes were directly formed on GCE via in-situ electrochemical reduction and polymerization processes step by step. The compositions, morphologies, and electrochemical properties of membranes were investigated with X-ray powder diffractometry (XRD), Fourier transform infrared spectrum (FTIR), Field emission scanning electron microscopy (FESEM) combined with various electrochemical methods. The fabricated electrochemical sensor labeled as GCEârGO-Au/MIPs exhibited excellent performance in determining of DMY under optimal experimental conditions. A wide linear detection range (LDR) ranges from 2.0x10.sup.-8 to 1.0x10.sup.-4 M together with a low limit of detection (LOD) of 1.2x10.sup.-8 M (S/N = 3) were achieved. Moreover, the electrochemical sensor was employed to determine DMY in real samples with satisfactory results.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Biology and Life Sciences / Chemical detectors / Chemical reduction / Chemical sensors / Chromatography / Design and construction / Electrochemical analysis / Electrochemical instruments / Electrochemistry / Electrodes / Electron microscopy / Engineering / Engineering and Technology / Field emission microscopy / Flavonoids / Forest products / Fourier transforms / Glassy carbon / Gold / Graphene / Identification and classification / Imprinted polymers / Infrared radiation / Laboratories / Membranes / Membranes (Technology) / Morphology / Nanocomposites / Nanoparticles / Pharmacokinetics / Pharmacology / Physical Sciences / Polymerization / Polymers / Polyvinyl alcohol / Quality control / Research and Analysis Methods / Scanning electron microscopy / Sensors / Social Sciences / Stability / Voltammetry