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Molecular characterization of a putative serine protease from Trichinella spiralis and its elicited immune protection
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Molecular characterization of a putative serine protease from Trichinella spiralis and its elicited immune protection
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Journal Article
Molecular characterization of a putative serine protease from Trichinella spiralis and its elicited immune protection
2018
Overview
In our previous work, a
Trichinella spiralis
putative serine protease (TsSP) was identified from ES products of
T. spiralis
intestinal infective larvae (IIL) and adult worms (AW) by immunoproteomics: it was highly expressed in IIL compared with muscle larvae (ML). In this study, the TsSP biological characteristics in larval invasion and growth were identified and its potential as a vaccine target against
Trichinella
infection were investigated. Expression of TsSP at various developmental phases (newborn larvae, ML, IIL, and AW) was detected by qPCR, immunofluorescent test and Western blotting. The rTsSP could specifically bind to the intestinal epithelial cell (IEC) membrane and enter into the cytoplasm. Anti-rTsSP serum suppressed the larval invasion of enterocytes in a dose-dependent mode, and killed newborn and ML of
T. spiralis
, decreased larval infectivity and development in the host by an ADCC-mediated mechanism. Immunization of mice with rTsSP produced a Th2 predominant immune response, and resulted in a 52.70% reduction of adult worms at 5 days post-infection (dpi) and a 52.10% reduction of muscle larvae at 42 dpi. The results revealed there was an interaction between TsSP and the host’s IEC; TsSP might be a pivotal protein for the invading, growing and parasiting of this nematode in the host. Vaccination of mice with rTsSP elicited immune protection, and TsSP is a potential target molecule for vaccines against enteral
Trichinella
infection.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Animals
/ Female
/ fluorescent antibody technique
/ Helminth Proteins - genetics
/ Helminth Proteins - immunology
/ Hogs
/ Larva - growth & development
/ Medicine
/ Mice
/ neonates
/ quantitative polymerase chain reaction
/ Serine
/ Serine Proteases - immunology
/ Trichinella spiralis - growth & development
/ Trichinella spiralis - physiology
/ vaccines
/ Veterinary Medicine/Veterinary Science
/ Virology
/ Zoonoses
