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Plasmodium falciparum msp1 and msp2 genetic diversity and allele frequencies in parasites isolated from symptomatic malaria patients in Bobo-Dioulasso, Burkina Faso
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Plasmodium falciparum msp1 and msp2 genetic diversity and allele frequencies in parasites isolated from symptomatic malaria patients in Bobo-Dioulasso, Burkina Faso
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Journal Article
Plasmodium falciparum msp1 and msp2 genetic diversity and allele frequencies in parasites isolated from symptomatic malaria patients in Bobo-Dioulasso, Burkina Faso
2018
Overview
Background
In Burkina Faso, malaria remains the overall leading cause of morbidity and mortality accounting for 35.12% of consultations, 40.83% of hospitalizations and 37.5% of deaths. Genotyping of malaria parasite populations remains an important tool to determine the types and number of parasite clones in an infection. The present study aimed to evaluate the merozoite surface protein 1 (
msp1
) and merozoite surface protein 2 (
msp2
) genetic diversity and allele frequencies in Bobo-Dioulasso, Burkina Faso.
Method
Dried blood spots (DBS) were collected at baseline from patients with uncomplicated malaria in urban health centers in Bobo-Dioulasso. Parasite DNA was extracted using chelex-100 and species were identified using nested PCR.
Plamodium falciparum msp1
and
msp2
genes were amplified by nested polymerase chain reaction (PCR) and PCR products were analyzed by electrophoresis on a 2.5% agarose gel. Alleles were categorized according to their molecular weight.
Results
A total of 228 blood samples were analyzed out of which 227 (99.9%) were confirmed as
P. falciparum-
positive and one sample classified as mixed infection for
P. malaria
and
P. falciparum.
In
msp1
, the K1 allelic family was predominant with 77.4% (162/209) followed respectively by the MAD20 allelic family with 41.3% and R033 allelic family with 36%. In
msp2
, the 3D7 allelic family was the most frequently detected with 93.1 % compared to FC27 with 41.3%. Twenty-one different alleles were observed in
msp1
with 9 alleles for K1, 8 alleles for MAD20 and 4 alleles for R033. In
msp2
, 25 individual alleles were detected with 10 alleles for FC27 and 15 alleles for 3D7. The mean multiplicity of
falciparum
infection was 1.95 with respectively 1.8 (1.76–1.83) and 2.1 (2.03–2.16) for
msp1
and
msp2
(
P
= 0.01).
Conclusions
Our study showed high genetic diversity and allelic frequencies of
msp1
and
msp2
in
Plasmodium falciparum
isolates from symptomatic malaria patients in Bobo-Dioulasso.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Adult
/ Agarose
/ Alleles
/ Antigens, Protozoan - genetics
/ Biomedical and Life Sciences
/ Blood
/ Child
/ Clones
/ DNA
/ family
/ Female
/ Gels
/ Humans
/ Infant
/ Malaria
/ Malaria, Falciparum - epidemiology
/ Malaria, Falciparum - parasitology
/ Male
/ Merozoite Surface Protein 1 - genetics
/ msp1
/ msp2
/ Patients
/ PCR
/ Plasmodium falciparum - genetics
/ Plasmodium falciparum - isolation & purification
/ Proteins
/ Protozoan Proteins - genetics
/ species
/ Veterinary Medicine/Veterinary Science
/ Virology
