Journal Article

NOD1 Activators Link Innate Immunity to Insulin Resistance

Liu, Zhi, Klip, Amira, Schertzer, Jonathan D., Fullerton, Morgan D., Steinberg, Gregory R., Giacca, Adria, Tamrakar, Akhilesh K., Philpott, Dana J., Magalhães, Joao G., Pereira, Sandra, Bilan, Philip J.

2011

Overview

Insulin resistance associates with chronic inflammation, and participatory elements of the immune system are emerging. We hypothesized that bacterial elements acting on distinct intracellular pattern recognition receptors of the innate immune system, such as bacterial peptidoglycan (PGN) acting on nucleotide oligomerization domain (NOD) proteins, contribute to insulin resistance. Metabolic and inflammatory properties were assessed in wild-type (WT) and NOD1/2(-/-) double knockout mice fed a high-fat diet (HFD) for 16 weeks. Insulin resistance was measured by hyperinsulinemic euglycemic clamps in mice injected with mimetics of meso-diaminopimelic acid-containing PGN or the minimal bioactive PGN motif, which activate NOD1 and NOD2, respectively. Systemic and tissue-specific inflammation was assessed using enzyme-linked immunosorbent assays in NOD ligand-injected mice. Cytokine secretion, glucose uptake, and insulin signaling were assessed in adipocytes and primary hepatocytes exposed to NOD ligands in vitro. NOD1/2(-/-) mice were protected from HFD-induced inflammation, lipid accumulation, and peripheral insulin intolerance. Conversely, direct activation of NOD1 protein caused insulin resistance. NOD1 ligands induced peripheral and hepatic insulin resistance within 6 h in WT, but not NOD1(-/-), mice. NOD2 ligands only modestly reduced peripheral glucose disposal. NOD1 ligand elicited minor changes in circulating proinflammatory mediators, yet caused adipose tissue inflammation and insulin resistance of muscle AS160 and liver FOXO1. Ex vivo, NOD1 ligand caused proinflammatory cytokine secretion and impaired insulin-stimulated glucose uptake directly in adipocytes. NOD1 ligand also caused inflammation and insulin resistance directly in primary hepatocytes from WT, but not NOD1(-/-), mice. We identify NOD proteins as innate immune components that are involved in diet-induced inflammation and insulin intolerance. Acute activation of NOD proteins by mimetics of bacterial PGNs causes whole-body insulin resistance, bolstering the concept that innate immune responses to distinctive bacterial cues directly lead to insulin resistance. Hence, NOD1 is a plausible, new link between innate immunity and metabolism.

• Publisher: American Diabetes Association
• Subject: Adipocytes / Adipose Tissue - immunology / Adipose Tissue - metabolism / Animals / Antibiotics / Avian Proteins - metabolism / Biological and medical sciences / Body fat / Cytokines / Cytokines - metabolism / Diabetes / Diabetes. Impaired glucose tolerance / Diet / Dietary Fats - metabolism / Endocrine pancreas. Apud cells (diseases) / Endocrinopathies / Etiopathogenesis. Screening. Investigations. Target tissue resistance / Forkhead Box Protein O1 / Forkhead Transcription Factors - metabolism / Glucose / Glucose Clamp Technique / Immune system / Immunity / Immunity (Physiology) / Immunity, Innate - physiology / Inflammation / Inflammation - immunology / Inflammation - metabolism / Insulin resistance / Insulin Resistance - immunology / Laboratories / Ligands / Medical sciences / Metabolism / Mice / Mice, Knockout / Nod1 Signaling Adaptor Protein - genetics / Nod1 Signaling Adaptor Protein - immunology / Nod1 Signaling Adaptor Protein - metabolism / Nod2 Signaling Adaptor Protein - genetics / Nod2 Signaling Adaptor Protein - immunology / Nod2 Signaling Adaptor Protein - metabolism / Pathogens / Pattern recognition / Pattern recognition receptors / Phosphorylation / Physiological aspects / Proteins / Research design / Risk factors / Sensors / Signal Transduction - immunology / Type 2 diabetes