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Effects of antibiotic treatment on the fecundity of Rhipicephalus haemaphysaloides ticks
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Effects of antibiotic treatment on the fecundity of Rhipicephalus haemaphysaloides ticks
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Journal Article
Effects of antibiotic treatment on the fecundity of Rhipicephalus haemaphysaloides ticks
2018
Overview
Background
Endosymbiotic bacteria inhabit a variety of arthropods including ticks and may have multiple effects on the host’s survival, reproduction or pathogen acquisition and transmission.
Rhipicephalus haemaphysaloides
is one of the most widely distributed tick species in China. The symbiotic bacteria composition and their impacts to
R. haemaphysaloides
ticks have not been studied. The present study investigated the composition of microbial community in
R. haemaphysaloides
ticks and then assessed the effects of endosymbionts on the host’s fecundity by antibiotic treatment experiments.
Methods
The microbial population of female and male
R. haemaphysaloides
ticks was analyzed using Illumina Miseq sequencing of
16S
rRNA gene. Thirty engorged female ticks were then randomly divided into five groups and injected with ampicillin, ciprofloxacin, kanamycin, tetracycline, or phosphate-buffered solution (PBS), respectively. Effects of antibiotic treatments on maternal oviposition, egg hatching and density of endosymbionts were evaluated.
Results
Illumina Miseq sequencing showed that
Coxiella
and
Rickettsia
were the predominant bacterial genera inhabiting
R. haemaphysaloides
ticks. Antibiotic treatment experiments found that kanamycin reduced the density of
Coxiella
-like endosymbiont (
Coxiella
-LE hereafter) in eggs, ciprofloxacin reduced the density of
Rickettsia
-like endosymbiont (
Rickettsia
-LE), and tetracycline had effect on both endosymbionts, while ampicillin affected neither. Meanwhile hatching rates of eggs were observed to decrease greatly in the kanamycin or tetracycline-treated group but maintained in the ampicillin or ciprofloxacin-treated group. Furthermore, the reduced hatching rates were found to be associated with density of
Coxiella
-LE in eggs.
Conclusions
The findings indicate that
Coxiella
-LE is essential for the reproduction of
R. haemaphysaloides
ticks, and that kanamycin can be used to study the role of
Coxiella
-LE on ticks.
Publisher
BioMed Central,BioMed Central Ltd,BMC
Subject
/ Analysis
/ Animals
/ Anti-Bacterial Agents - administration & dosage
/ Anti-Bacterial Agents - adverse effects
/ Anti-Bacterial Agents - therapeutic use
/ Biomedical and Life Sciences
/ China
/ Coxiella
/ eggs
/ Female
/ females
/ genes
/ Male
/ males
/ Microbial Consortia - drug effects
/ Microbial Consortia - genetics
/ Rhipicephalus - drug effects
/ Rhipicephalus - microbiology
/ Rhipicephalus haemaphysaloides
/ RNA, Ribosomal, 16S - genetics
/ species
/ ticks
/ Veterinary Medicine/Veterinary Science
/ Virology
