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Egfr signaling promotes juvenile hormone biosynthesis in the German cockroach
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Egfr signaling promotes juvenile hormone biosynthesis in the German cockroach
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Journal Article
Egfr signaling promotes juvenile hormone biosynthesis in the German cockroach
2022
Overview
Background
In insects, an interplay between the activities of distinct hormones, such as juvenile hormone (JH) and 20-hydroxyecdysone (20E), regulates the progression through numerous life history hallmarks. As a crucial endocrine factor, JH is mainly synthesized in the corpora allata (CA) to regulate multiple physiological and developmental processes, including molting, metamorphosis, and reproduction. During the last century, significant progress has been achieved in elucidating the JH signal transduction pathway, while less progress has been made in dissecting the regulatory mechanism of JH biosynthesis. Previous work has shown that receptor tyrosine kinase (RTK) signaling regulates hormone biosynthesis in both insects and mammals. Here, we performed a systematic RNA interference (RNAi) screening to identify RTKs involved in regulating JH biosynthesis in the CA of adult
Blattella germanica
females.
Results
We found that the epidermal growth factor receptor (Egfr) is required for promoting JH biosynthesis in the CA of adult females. The Egf ligands Vein and Spitz activate Egfr, followed by Ras/Raf/ERK signaling, and finally activation of the downstream transcription factor Pointed (Pnt). Importantly, Pnt induces the transcriptional expression of two key enzyme-encoding genes in the JH biosynthesis pathway: juvenile hormone acid methyltransferase (JHAMT) and methyl farnesoate epoxidase (CYP15A1). Dual-luciferase reporter assay shows that Pnt is able to activate a promoter region of
Jhamt
. In addition, electrophoretic mobility shift assay confirms that Pnt directly binds to the − 941~ − 886 nt region of the
Jhamt
promoter.
Conclusions
This study reveals the detailed molecular mechanism of Egfr signaling in promoting JH biosynthesis in the German cockroach, shedding light on the intricate regulation of JH biosynthesis during insect development.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Animals
/ Biomedical and Life Sciences
/ Enzymes
/ Epidermal growth factor receptors
/ Extracellular signal-regulated kinase
/ Female
/ Females
/ Genes
/ Genomes
/ Hormones
/ Insects
/ JHAMT
/ Juvenile Hormones - metabolism
/ Kinases
/ Ligands
/ Mammals
/ Molting
/ Ovaries
/ Pnt
/ Protein-tyrosine kinase receptors
/ Proteins
/ Regulatory mechanisms (biology)
/ Signal Transduction - physiology
/ Tyrosine
