Journal Article

Comparative analysis of antibody- and lipid-based multiplexing methods for single-cell RNA-seq

Movahedi, Kiavash, Aerts, Stein, Van Houdt, Jeroen, Poovathingal, Suresh, Scheyltjens, Isabelle, Wils, Hans, Reumers, Joke, Van den Hoecke, Silvie, Mylka, Viacheslav, Hulselmans, Gert, Seurinck, Ruth, Matetovici, Irina, Verstaen, Kevin, Vandamme, Niels, Saeys, Yvan, Aerts, Jeroen

2022

Overview

Background Multiplexing of samples in single-cell RNA-seq studies allows a significant reduction of the experimental costs, straightforward identification of doublets, increased cell throughput, and reduction of sample-specific batch effects. Recently published multiplexing techniques using oligo-conjugated antibodies or -lipids allow barcoding sample-specific cells, a process called “hashing.” Results Here, we compare the hashing performance of TotalSeq-A and -C antibodies, custom synthesized lipids and MULTI-seq lipid hashes in four cell lines, both for single-cell RNA-seq and single-nucleus RNA-seq. We also compare TotalSeq-B antibodies with CellPlex reagents (10x Genomics) on human PBMCs and TotalSeq-B with different lipids on primary mouse tissues. Hashing efficiency was evaluated using the intrinsic genetic variation of the cell lines and mouse strains. Antibody hashing was further evaluated on clinical samples using PBMCs from healthy and SARS-CoV-2 infected patients, where we demonstrate a more affordable approach for large single-cell sequencing clinical studies, while simultaneously reducing batch effects. Conclusions Benchmarking of different hashing strategies and computational pipelines indicates that correct demultiplexing can be achieved with both lipid- and antibody-hashed human cells and nuclei, with MULTISeqDemux as the preferred demultiplexing function and antibody-based hashing as the most efficient protocol on cells. On nuclei datasets, lipid hashing delivers the best results. Lipid hashing also outperforms antibodies on cells isolated from mouse brain. However, antibodies demonstrate better results on tissues like spleen or lung.

• Publisher: BioMed Central,Springer Nature B.V,BMC
• Subject: Accuracy / Animal Genetics and Genomics / Animals / Annotations / Antibodies / Antibodies - chemistry / Antigens / Bioinformatics / Biomedical and Life Sciences / brain / Cancer / Case-Control Studies / Cell Line, Tumor / Cell lines / Cell Nucleus - chemistry / CITE-seq / Classification / Comparative analysis / Computational neuroscience / COVID-19 - blood / data collection / Evolutionary Biology / Experiments / Flow cytometry / Genetic diversity / genetic variation / genome / Genomics / Hashing / Human Genetics / Humans / Life Sciences / Lipids / Lipids - chemistry / lungs / Methods / Mice / Mice, Inbred BALB C / Mice, Inbred C57BL / Microbial Genetics and Genomics / MULTI-seq / Neutrophils - chemistry / Neutrophils - immunology / Neutrophils - virology / Plant Genetics and Genomics / Ribonucleic acid / RNA / Sample multiplexing / scRNA-seq / sequence analysis / Sequence Analysis, RNA - methods / Severe acute respiratory syndrome coronavirus 2 / Single-Cell Analysis - methods / Spleen