Journal Article

Zika virus evolution and spread in the Americas

Grubaugh, Nathan D., Gnirke, Andreas, Robles-Sikisaka, Refugio, Rojas, Diana P., Wohl, Shirlee, Brown, Catherine M., Bozza, Fernando A., Qu, James, Winnicki, Sarah M., Isern, Sharon, Delatorre, Edson, Tomkins-Tinch, Christopher H., Anzinger, Joshua J., Barcellona, Carolyn M., Shah, Rickey R., Villar, Luis, Yozwiak, Nathan L., Gladden-Young, Adrianne, Mattar, Salim, Sabeti, Pardis C., Hennigan, Scott, Cerbino-Neto, Jose, Cannons, Andrew C., Michael, Scott F., Ramírez, Rosa M. Gélvez, Vieira, Yasmine R., Oliveira, Glenn, Hogan, Kelly N., Andersen, Kristian G., Barbosa-Lima, Giselle, Gangavarapu, Karthik, Parham, Leda A., Luo, Cynthia Y., Sabina, Brandon, Souza, Thiago M. L., Vasquez, Chalmers, Garcia, Kimberly F., Bozza, Patricia T., Cone, Marshall R., Porcelli, Mario C., Matranga, Christian B., Bosch, Irene, Kopp, Edgar W., Paul, Lauren M., Gehrke, Lee, Schaffner, Stephen F., Smole, Sandra, Baniecki, Mary Lynn, Scotland, Sarah, Rambaut, Andrew, Lin, Aaron E., Tan, Amanda L., Lorenzana, Ivette, Park, Daniel J., Halloran, M. Elizabeth, Barnes, Kayla G., West, Kendra, Degrave, Wim, Metsky, Hayden C., Vali

2017

Overview

One hundred and ten Zika virus genomes from ten countries and territories involved in the Zika virus epidemic reveal rapid expansion of the epidemic within Brazil and multiple introductions to other regions. Zika epidemiology Three papers in this issue present a wealth of new Zika virus (ZIKV) genome sequences and further insights into the genetic epidemiology of ZIKV. Nathan Grubaugh et al . provide 39 new ZIKV genome sequences from infected patients and Aedes aegypti mosquitoes in Florida. Phylogenetic analysis suggests that the virus has been introduced on multiple separate occasions, probably linked to travel from the Caribbean. They find a low probability of long-term persistence of ZIKV transmission chains within Florida, suggesting that the potential for future ZIKV outbreaks there will depend on transmission dynamics in the Americas. Nuno Faria et al . and Hayden Metsky et al . reconstruct the spread of ZIKV in Brazil and the Americas. Faria et al . provide 54 new ZIKV genomes, several sequenced in real time in a mobile genomics laboratory. They trace the spatial origins and spread of ZIKV in Brazil and the Americas and date the timing of the international spread of ZIKV from Brazil. They find that northeast Brazil had a crucial role in the establishment of the epidemic and the spread of the virus within Brazil and the Americas. Metsky et al . generate 110 ZIKV genomes from clinical and mosquito samples from ten regions. They also see rapid expansion of the epidemic within Brazil and multiple introductions to other geographic areas. In agreement with Faria et al ., they find that ZIKV circulated unobserved for many months before transmission was detected. Metsky et al . additionally describe ZIKV evolution and discuss how the accumulation of mutations might affect the performance of diagnostic tests in the future. Although the recent Zika virus (ZIKV) epidemic in the Americas and its link to birth defects have attracted a great deal of attention 1 , 2 , much remains unknown about ZIKV disease epidemiology and ZIKV evolution, in part owing to a lack of genomic data. Here we address this gap in knowledge by using multiple sequencing approaches to generate 110 ZIKV genomes from clinical and mosquito samples from 10 countries and territories, greatly expanding the observed viral genetic diversity from this outbreak. We analysed the timing and patterns of introductions into distinct geographic regions; our phylogenetic evidence suggests rapid expansion of the outbreak in Brazil and multiple introductions of outbreak strains into Puerto Rico, Honduras, Colombia, other Caribbean islands, and the continental United States. We find that ZIKV circulated undetected in multiple regions for many months before the first locally transmitted cases were confirmed, highlighting the importance of surveillance of viral infections. We identify mutations with possible functional implications for ZIKV biology and pathogenesis, as well as those that might be relevant to the effectiveness of diagnostic tests.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 45/77 / 45/90 / 45/91 / 631/208/212/2306 / 631/208/514/1949 / Animals / Aquatic insects / Bioinformatics / Birth defects / Brazil - epidemiology / Colombia - epidemiology / Congenital defects / Culicidae - virology / Dengue fever / Disease Outbreaks - statistics & numerical data / Distribution / Epidemiology / Evolution / Genetic diversity / Genome, Viral - genetics / Genomes / Geographic Mapping / Honduras - epidemiology / Humanities and Social Sciences / Humans / letter / Metagenome - genetics / Molecular Epidemiology / Mosquito Vectors - virology / Mosquitoes / multidisciplinary / Multivariate analysis / Mutation / Natural history / Outbreaks / Pathogenesis / Phylogenetics / Phylogeny / Public Health Surveillance / Puerto Rico - epidemiology / Science / Software / United States - epidemiology / Urine / Vector-borne diseases / Viral infections / Viruses / Zika virus / Zika Virus - classification / Zika Virus - genetics / Zika Virus - isolation & purification / Zika Virus - pathogenicity / Zika virus infection / Zika Virus Infection - diagnosis / Zika Virus Infection - epidemiology / Zika Virus Infection - transmission / Zika Virus Infection - virology