Journal Article

Multi-omic approach to characterize the venom of the parasitic wasp Cotesia congregata (Hymenoptera: Braconidae)

Tomas, Daniel, Boulain, Hélène, Gauthier, Jérémy, Moreau, Sébastien J. M., Labas, Valérie, Marchal, Lorène, Drezen, Jean-Michel, Musset, Karine

2025

Overview

Background Cotesia congregata is a parasitoid Hymenoptera belonging to the Braconidae family and carrying CCBV ( Cotesia congregata Bracovirus), an endosymbiotic polydnavirus. CCBV virus is considered as the main virulence factor of this species, which has raised questions, over the past thirty years, about the potential roles of venom in the parasitic interaction between C. congregata and its host, Manduca sexta (Lepidoptera: Sphingidae). To investigate C. congregata venom composition, we identified genes overexpressed in the venom glands (VGs) compared to ovaries, analyzed the protein composition of this fluid and performed a detailed analysis of conserved domains of these proteins. Results Of the 14 140 known genes of the C. congregata genome, 659 genes were significantly over-expressed (with 10-fold or higher changes in expression) in the VGs of female C. congregata , compared with the ovaries. We identified 30 proteins whose presence was confirmed in venom extracts by proteomic analyses. Twenty-four of these were produced as precursor molecules containing a predicted signal peptide. Six of the proteins lacked a predicted signal peptide, suggesting that venom production in C. congregata also involves non-canonical secretion mechanisms. We have also analysed 18 additional proteins and peptides of interest whose presence in venom remains uncertain, but which could play a role in VG function. Conclusions Our results show that the venom of C. congregata not only contains proteins (including several enzymes) homologous to well-known venomous compounds, but also original proteins that appear to be specific to this species. This exhaustive study sheds a new light on this venom composition, the molecular diversity of which was unexpected. These data pave the way for targeted functional analyses and to better understand the evolutionary mechanisms that have led to the formation of the venomous arsenals we observe today in parasitoid insects.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Animal biology / Animal Genetics and Genomics / Animals / Biochemistry, Molecular Biology / Biomedical and Life Sciences / Braconidae / Composition / Cotesia / Cotesia congregata / Extracellular vesicles / Female / Females / Genes / Genetic aspects / Genetic research / Genomes / Genomics / Hymenoptera / Insect Proteins - chemistry / Insect Proteins - genetics / Insect Proteins - metabolism / Insects / Invertebrate Zoology / Life Sciences / Microarrays / Microbial Genetics and Genomics / Microscopy / Multiomics / Omics of Parasites 2 / Ovaries / Peptides / Physiological aspects / Physiology / Plant Genetics and Genomics / Protein composition / Proteins / Proteomics / Proteomics - methods / Proteomics; Braconidae; Parasitoid / Success / Transcriptomics / Venom / Venom gland / Virulence / Virulence factors / Viruses / Wasp Venoms - chemistry / Wasp Venoms - genetics / Wasp Venoms - metabolism / Wasps / Wasps - genetics / Wasps - metabolism / Wasps - virology