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An ES-Like Pluripotent State in FGF-Dependent Murine iPS cells
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An ES-Like Pluripotent State in FGF-Dependent Murine iPS cells
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An ES-Like Pluripotent State in FGF-Dependent Murine iPS cells
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Journal Article
An ES-Like Pluripotent State in FGF-Dependent Murine iPS cells
2010
Overview
Recent data demonstrates that stem cells can exist in two morphologically, molecularly and functionally distinct pluripotent states; a naïve LIF-dependent pluripotent state which is represented by murine embryonic stem cells (mESCs) and an FGF-dependent primed pluripotent state represented by murine and rat epiblast stem cells (EpiSCs). We find that derivation of induced pluripotent stem cells (iPSCs) under EpiSC culture conditions yields FGF-dependent iPSCs from hereon called FGF-iPSCs) which, unexpectedly, display naïve ES-like/ICM properties. FGF-iPSCs display X-chromosome activation, multi-lineage differentiation, teratoma competence and chimera contribution in vivo. Our findings suggest that in 129 and Bl6 mouse strains, iPSCs can dominantly adopt a naive pluripotent state regardless of culture growth factor conditions. Characterization of the key molecular signalling pathways revealed FGF-iPSCs to depend on the Activin/Nodal and FGF pathways, while signalling through the JAK-STAT pathway is not required for FGF-iPS cell maintenance. Our findings suggest that in 129 and Bl6 mouse strains, iPSCs can dominantly adopt a naive pluripotent state regardless of culture growth factor conditions.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Animals
/ Biology
/ Embryonic Stem Cells - cytology
/ Embryos
/ Fibroblast Growth Factors - metabolism
/ Genetics
/ Genomics
/ Green Fluorescent Proteins - metabolism
/ Induced Pluripotent Stem Cells - cytology
/ Intercellular Signaling Peptides and Proteins - metabolism
/ Kinases
/ Medicine
/ Mice
/ Teratoma
/ Xenopus
