Journal Article

PP13, Maternal ABO Blood Groups and the Risk Assessment of Pregnancy Complications

Tarquini, Federica, Meiri, Hamutal, Kovalszky, Ilona, Dong, Zhong, Kim, Chong Jai, Xu, Yi, Romero, Roberto, Sammar, Marei, Than, Nandor Gabor, Papp, Zoltan, Ackerman, Ron, Erez, Offer, Szilagyi, Andras, Fule, Tibor, Karaszi, Katalin, Zavodszky, Peter, Gonen, Ron, Barna, Laszlo

2011

Overview

Placental Protein 13 (PP13), an early biomarker of preeclampsia, is a placenta-specific galectin that binds beta-galactosides, building-blocks of ABO blood-group antigens, possibly affecting its bioavailability in blood. We studied PP13-binding to erythrocytes, maternal blood-group effect on serum PP13 and its performance as a predictor of preeclampsia and intrauterine growth restriction (IUGR). Datasets of maternal serum PP13 in Caucasian (n = 1078) and Hispanic (n = 242) women were analyzed according to blood groups. In vivo, in vitro and in silico PP13-binding to ABO blood-group antigens and erythrocytes were studied by PP13-immunostainings of placental tissue-microarrays, flow-cytometry of erythrocyte-bound PP13, and model-building of PP13--blood-group H antigen complex, respectively. Women with blood group AB had the lowest serum PP13 in the first trimester, while those with blood group B had the highest PP13 throughout pregnancy. In accordance, PP13-binding was the strongest to blood-group AB erythrocytes and weakest to blood-group B erythrocytes. PP13-staining of maternal and fetal erythrocytes was revealed, and a plausible molecular model of PP13 complexed with blood-group H antigen was built. Adjustment of PP13 MoMs to maternal ABO blood group improved the prediction accuracy of first trimester maternal serum PP13 MoMs for preeclampsia and IUGR. ABO blood group can alter PP13-bioavailability in blood, and it may also be a key determinant for other lectins' bioavailability in the circulation. The adjustment of PP13 MoMs to ABO blood group improves the predictive accuracy of this test.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: ABO Blood-Group System - chemistry / ABO Blood-Group System - metabolism / ABO system / Amino Acid Sequence / Antigens / Binding / Bioavailability / Biology / Biomarkers / Blood / Blood circulation / Blood group AB / Blood group B / Blood groups / Breweries / Cancer / Complications / Computer Science / Cytometry / Erythrocytes / Erythrocytes - metabolism / European Continental Ancestry Group / Female / Fetal Growth Retardation - blood / Fetal Growth Retardation - diagnosis / Fetuses / Galactosides / Galectins - blood / Galectins - chemistry / Galectins - metabolism / Gangrene / Gynecology / H antigen / Health risk assessment / Hispanic Americans / Humans / Hypertension / In vivo methods and tests / Laboratories / Lectins / Mathematical models / Medical research / Medicine / Models, Molecular / Molecular Sequence Data / Mothers / Obstetrics / Oxidative stress / Pathology / Placenta / Pre-eclampsia / Pre-Eclampsia - blood / Pre-Eclampsia - diagnosis / Predictions / Predictive Value of Tests / Preeclampsia / Pregnancy / Pregnancy complications / Pregnancy Complications - blood / Pregnancy Complications - diagnosis / Pregnancy Complications - metabolism / Pregnancy Proteins - blood / Pregnancy Proteins - chemistry / Pregnancy Proteins - metabolism / Pregnant women / Prenatal development / Protein Conformation / Proteins / Regulation / Risk Assessment / Substrate Specificity / Womens health