Journal Article

Preventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertension

Junior, Luis Antonio Justulin, Pacagnelli, Francis Lopes, Aguiar, Andreo Fernando, de Souza Castilho, Anthony César, Cury, Sarah Santiloni, Mariano, Thaoan Bruno, Okoshi, Katashi, Cicogna, Antonio Carlos, Carvalho, Robson Francisco, Dias, Raisa de Jesus Dutra, de Almeida Sabela, Ana Karenina Dias, de Oliveira, André Casanova

2021

Overview

To gain insight on the impact of preventive exercise during pulmonary arterial hypertension (PAH), we evaluated the gene expression of myosins and gene-encoding proteins associated with the extracellular matrix remodeling of right hypertrophied ventricles. We used 32 male Wistar rats, separated in four groups: Sedentary Control (S, n = 8); Control with Training (T, n = 8); Sedentary with Pulmonary Arterial Hypertension (SPAH, n = 8); and Pulmonary Arterial Hypertension with Training (TPAH, n = 8). All rats underwent a two-week adaptation period; T and TPAH group rats then proceeded to an eight-week training period on a treadmill. At the beginning of the 11th week, S and T groups received an intraperitoneal injection of saline, and SPAH and TPAH groups received an injection of monocrotaline (60 mg/kg). Rats in the T and TPAH groups then continued with the training protocol until the 13th week. We assessed exercise capacity, echocardiography analysis, Fulton’s index, cross-sectional areas of cardiomyocytes, collagen content and types, and fractal dimension (FD). Transcript abundance of myosins and extracellular matrix genes were estimated through reverse transcription-quantitative PCR (RT-qPCR). When compared to the SPAH group, the TPAH group showed increases in functional capacity and pulmonary artery acceleration time/pulmonary ejection time ratio and decreases in Fulton’s index and cross-sectional areas of myocyte cells. However, preventive exercise did not induce alterations in col1a1 and myh7 gene expression. Our findings demonstrate that preventive exercise improved functional capacity, reduced cardiac hypertrophy, and attenuated PH development without interfering in mRNA-encoding myosin and collagen expression during PAH.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Adaptation / Aerobics / Analysis / Animal sciences / Animals / Biology / Biology and Life Sciences / Cardiomyocytes / Collagen / Collagen (type I) / Collagen - genetics / Collagen - metabolism / Echocardiography / Exercise / Extracellular matrix / Fitness equipment / Fractal geometry / Gene expression / Gene Expression Regulation / Genetic aspects / Health aspects / Heart failure / Hypertension / Hypertension, Pulmonary - genetics / Hypertension, Pulmonary - metabolism / Hypertension, Pulmonary - prevention & control / Hypertrophy / Hypertrophy, Right Ventricular - genetics / Hypertrophy, Right Ventricular - metabolism / Hypertrophy, Right Ventricular - prevention & control / Injection / Internal medicine / Laboratory animals / Male / Medicine and Health Sciences / Messenger RNA / Monocrotaline / MYH7 gene / Myocytes / Myosin / Myosins - genetics / Myosins - metabolism / Physical Conditioning, Animal / Physical fitness / Physical therapy / Pulmonary Arterial Hypertension - genetics / Pulmonary Arterial Hypertension - metabolism / Pulmonary artery / Pulmonary hypertension / Rats / Rats, Wistar / Reverse transcription / RNA, Messenger - genetics / RNA, Messenger - metabolism / Sedentary behavior / Social Sciences / Training / Treadmills / Velocity / Zoology