Journal Article

Comparison of Chemical Sensitivity of Fresh and Long-Stored Heat Resistant Neosartorya fischeri Environmental Isolates Using BIOLOG Phenotype MicroArray System

Frąc, Magdalena, Panek, Jacek, Bilińska-Wielgus, Nina

2016

Overview

Spoilage of heat processed food and beverage by heat resistant fungi (HRF) is a major problem for food industry in many countries. Neosartorya fischeri is the leading source of spoilage in thermally processed products. Its resistance to heat processing and toxigenicity makes studies about Neosartorya fischeri metabolism and chemical sensitivity essential. In this study chemical sensitivity of two environmental Neosartorya fischeri isolates were compared. One was isolated from canned apples in 1923 (DSM3700), the other from thermal processed strawberry product in 2012 (KC179765), used as long-stored and fresh isolate, respectively. The study was conducted using Biolog Phenotype MicroArray platforms of chemical sensitivity panel and traditional hole-plate method. The study allowed for obtaining data about Neosartorya fischeri growth inhibitors. The fresh isolate appeared to be much more resistant to chemical agents than the long-stored isolate. Based on phenotype microarray assay nitrogen compounds, toxic cations and membrane function compounds were the most effective in growth inhibition of N. fischeri isolates. According to the study zaragozic acid A, thallium(I) acetate and sodium selenate were potent and promising N. fischeri oriented fungicides which was confirmed by both chemical sensitivity microplates panel and traditional hole-plate methods.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Acetic acid / Antibiotics / Antifungal agents / Antimicrobial agents / Apples / Biology and Life Sciences / Cations / Chemical agents / Chemicals / DNA microarrays / Fluorides / Food industry / Food Microbiology / Food processing / Food processing industry / Food spoilage / Fungi / Fungicides / Genetic aspects / Genotype & phenotype / Heat / Hot Temperature / Laboratories / Laboratory equipment / Medicine and Health Sciences / Metabolism / Microbial genetics / Neosartorya - drug effects / Neosartorya - growth & development / Neosartorya - metabolism / Neosartorya fischeri / Nitrogen compounds / Phenotype / Physical Sciences / Physiological aspects / Processed foods / Sensitivity / Sodium / Sodium selenate / Spoilage / Spores, Fungal - drug effects / Spores, Fungal - growth & development / Spores, Fungal - metabolism / Studies / Thallium / Toxigenicity