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Antibiotics induced intestinal tight junction barrier dysfunction is associated with microbiota dysbiosis, activated NLRP3 inflammasome and autophagy
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Antibiotics induced intestinal tight junction barrier dysfunction is associated with microbiota dysbiosis, activated NLRP3 inflammasome and autophagy
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Journal Article
Antibiotics induced intestinal tight junction barrier dysfunction is associated with microbiota dysbiosis, activated NLRP3 inflammasome and autophagy
2019
Overview
Tight junction barrier is critical to intestinal homeostasis. Applying antibiotics to treat infections is common in clinical practice, which may affect intestinal microbiota. Intestinal microbiota dysbiosis is involved in the occurrence of some gastrointestinal diseases. Therefore, this study was aimed to investigate the influence of antibiotics on intestinal tight junction barrier and the possible underlying mechanisms. Healthy adult female C57BL/6 mice were treated with a broad-spectrum antibiotic cocktail for 14 days. 16S rDNA Illumina sequencing and headspace gas chromatography-mass spectrometry (HS-GC/MS) were respectively used to analyze microbial community and to detect short-chain fatty acids (SCFAs) contents. In vivo intestinal paracellular permeability to fluorescein isothiocyanate-dextran (FITC-dextran) was measured. Protein expression was determined by immunoblotting. Immunofluoresence was applied to observe the distributions of ZO-1, LC3B and ASC. Antibiotics remarkably altered intestinal microbiota composition in healthy mice, accompanying reduced SCFAs' concentrations. In addition, the intestinal tight junction barrier was disrupted by antibiotic treatment, as evidenced by increased intestinal paracellular permeability to FITC-dextran, decreased tight junction protein expressions, and disrupted ZO-1 morphology. Furthermore, NLRP3 inflammasome and autophagy were activated by antibiotic treatment. In conclusion, intestinal epithelial tight junction barrier dysfunction induced by antibiotics is associated with intestinal microbiota dysbiosis, activated NLRP3 inflammasome and autophagy in mice.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Animals
/ Anti-Bacterial Agents - adverse effects
/ Anti-Bacterial Agents - pharmacology
/ Burns
/ Complications and side effects
/ Dextran
/ Dextrans
/ Dysbiosis - chemically induced
/ Fatty Acids, Volatile - metabolism
/ Fluorescein-5-isothiocyanate - analogs & derivatives
/ Fluorescein-5-isothiocyanate - chemistry
/ Gene Expression Regulation - drug effects
/ Humans
/ Inflammasomes - drug effects
/ Ischemia
/ Medicine and Health Sciences
/ Mice
/ Microbiota (Symbiotic organisms)
/ NLR Family, Pyrin Domain-Containing 3 Protein - genetics
/ Proteins
/ rRNA 16S
/ Tight Junctions - drug effects
/ Tight Junctions - microbiology
/ Trauma
