Journal Article

iTRAQ-Based Quantitative Proteomic Analysis of Cotton Roots and Leaves Reveals Pathways Associated with Salt Stress

Yuan, Youlu, Liu, Shaodong, Peng, Jun, Chen, Tingting, Shi, Yuzhen, Li, Junwen, Ma, Huijuan, Liu, Aiying, Zhang, Lei, Gong, Wankui, Gong, Juwu, Ge, Qun, Zhao, Xinhua, Shang, Haihong, Zhang, Siping

2016

Overview

Salinity is a major abiotic stress that affects plant growth and development. In this study, we performed a proteomic analysis of cotton roots and leaf tissue following exposure to saline stress. 611 and 1477 proteins were differentially expressed in the roots and leaves, respectively. In the roots, 259 (42%) proteins were up-regulated and 352 (58%) were down-regulated. In the leaves, 748 (51%) proteins were up-regulated and 729 (49%) were down-regulated. On the basis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, we concluded that the phenylalanine metabolism and starch and sucrose metabolism were active for energy homeostasis to cope with salt stress in cotton roots. Moreover, photosynthesis, pyruvate metabolism, glycolysis / gluconeogenesis, carbon fixation in photosynthetic organisms and phenylalanine metabolism were inhabited to reduce energy consumption. Characterization of the signaling pathways will help elucidate the mechanism activated by cotton in response to salt stress.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Abiotic stress / Biochemistry / Biology / Biology and Life Sciences / Biosynthesis / Carbohydrate metabolism / Carbon fixation / Chen, Lei / Cotton / Ecology and Environmental Sciences / Encyclopedias / Energy balance / Energy consumption / Energy metabolism / Enzymes / Gene Expression Regulation, Plant / Genetics / Genomes / Gluconeogenesis / Glucose metabolism / Glycolysis / Gossypium - genetics / Gossypium - metabolism / Gossypium hirsutum / Homeostasis / Laboratories / Leaves / Lignin / Metabolism / Osmotic Pressure / Oxidative stress / Phenylalanine / Photosynthesis / Physical Sciences / Physiological aspects / Physiology / Plant growth / Plant Leaves - genetics / Plant Leaves - metabolism / Plant Proteins - biosynthesis / Plant Proteins - genetics / Plant Roots - genetics / Plant Roots - metabolism / Plant sciences / Plant tissues / Proteins / Proteomics / Proteomics - methods / Pyruvic acid / Roots / Salinity / Salt / Salts / Starch / Stresses / Sucrose / Sugar