Journal Article

Toll-Like Receptor 11 (TLR11) Interacts with Flagellin and Profilin through Disparate Mechanisms

Hayden, Matthew S., Hatai, Hirotsugu, Lepelley, Alice, Ghosh, Sankar, Zeng, Wangyong

2016

Overview

Toll-like receptors (TLRs) are innate immune receptors that sense a variety of pathogen-associated molecular patterns (PAMPs) by interacting with them and subsequently initiating signal transduction cascades that elicit immune responses. TLR11 has been shown to interact with two known protein PAMPs: Salmonella and E. coli flagellin FliC and Toxoplasma gondii profilin-like protein. Given the highly divergent biology of these pathogens recognized by TLR11, it is unclear whether common mechanisms are used to recognize these distinct protein PAMPs. Here we show that TLR11 interacts with these two PAMPs using different receptor domains. Furthermore, TLR11 binding to flagellin and profilin exhibits differential dependency on pH and receptor ectodomain cleavage.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Analysis / Animals / Biology and Life Sciences / Blotting, Western / Cascades / Cathepsins - metabolism / Cellular signal transduction / Cloning / Cloning, Molecular / Deoxyribonucleic acid / Dermatology / DNA / E coli / Flagellin / Flagellin - metabolism / Gene expression / HEK293 Cells / Humans / Immune response / Immune system / Immunology / Infections / Infectious diseases / Ligands / Mammals / Medicine and Health Sciences / Mice / Mutagenesis / Parasites / Pathogens / pH effects / Profilin / Profilins - metabolism / Protein Interaction Domains and Motifs / Proteins / Receptors / Recombinant Proteins / Research and Analysis Methods / Salmonella / Signal transduction / Surgeons / Toll-like receptors / Toll-Like Receptors - immunology / Toxoplasma / Toxoplasma gondii / Western blotting