Asset Details
Do you wish to reserve the book?
Proteome Analysis and Serological Characterization of Surface-Exposed Proteins of Rickettsia heilongjiangensis
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Proteome Analysis and Serological Characterization of Surface-Exposed Proteins of Rickettsia heilongjiangensis
Do you wish to request the book?
Proteome Analysis and Serological Characterization of Surface-Exposed Proteins of Rickettsia heilongjiangensis
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Proteome Analysis and Serological Characterization of Surface-Exposed Proteins of Rickettsia heilongjiangensis
2013
Overview
Rickettsia heilongjiangensis, the agent of Far-Eastern spotted fever (FESF), is an obligate intracellular bacterium. The surface-exposed proteins (SEPs) of rickettsiae are involved in rickettsial adherence to and invasion of host cells, intracellular bacterial growth, and/or interaction with immune cells. They are also potential molecular candidates for the development of diagnostic reagents and vaccines against rickettsiosis.
R. heilongjiangensis SEPs were identified by biotin-streptavidin affinity purification and 2D electrophoreses coupled with ESI-MS/MS. Recombinant SEPs were probed with various sera to analyze their serological characteristics using a protein microarray and an enzyme-linked immune sorbent assay (ELISA).
Twenty-five SEPs were identified, most of which were predicted to reside on the surface of R. heilongjiangensis cells. Bioinformatics analysis suggests that these proteins could be involved in bacterial pathogenesis. Eleven of the 25 SEPs were recognized as major seroreactive antigens by sera from R. heilongjiangensis-infected mice and FESF patients. Among the major seroreactive SEPs, microarray assays and/or ELISAs revealed that GroEL, OmpA-2, OmpB-3, PrsA, RplY, RpsB, SurA and YbgF had modest sensitivity and specificity for recognizing R. heilongjiangensis infection and/or spotted fever.
Many of the SEPs identified herein have potentially important roles in R. heilongjiangensis pathogenicity. Some of them have potential as serodiagnostic antigens or as subunit vaccine antigens against the disease.
Publisher
Public Library of Science,Public Library of Science (PLoS)
Subject
/ Animals
/ Antibodies, Bacterial - blood
/ Antibodies, Bacterial - metabolism
/ Antigens
/ Bacteria
/ Bacterial Outer Membrane Proteins - chemistry
/ Bacterial Outer Membrane Proteins - genetics
/ Bacterial Outer Membrane Proteins - immunology
/ Bacterial Outer Membrane Proteins - isolation & purification
/ Biology
/ Biotin
/ Electrophoresis, Polyacrylamide Gel
/ Enzyme-Linked Immunosorbent Assay
/ Fever
/ Humans
/ Male
/ Medicine
/ Mice
/ Proteins
/ Proteome
/ Reagents
/ Recombinant Proteins - genetics
/ Recombinant Proteins - metabolism
/ Sorbents
/ Vaccines
