Journal Article

Resveratrol Induces Premature Senescence in Lung Cancer Cells via ROS-Mediated DNA Damage

Wargovich, Michael J., Wang, Gavin Y., Luo, Hongmei, Yang, Aimin, Schulte, Bradley A.

2013

Overview

Resveratrol (RV) is a natural component of red wine and grapes that has been shown to be a potential chemopreventive and anticancer agent. However, the molecular mechanisms underlying RV's anticancer and chemopreventive effects are incompletely understood. Here we show that RV treatment inhibits the clonogenic growth of non-small cell lung cancer (NSCLC) cells in a dose-dependent manner. Interestingly, the tumor-suppressive effect of low dose RV was not associated with any significant changes in the expression of cleaved PARP and activated caspase-3, suggesting that low dose RV treatment may suppress tumor cell growth via an apoptosis-independent mechanism. Subsequent studies reveal that low dose RV treatment induces a significant increase in senescence-associated β-galactosidase (SA-β-gal) staining and elevated expression of p53 and p21 in NSCLC cells. Furthermore, we show that RV-induced suppression of lung cancer cell growth is associated with a decrease in the expression of EF1A. These results suggest that RV may exert its anticancer and chemopreventive effects through the induction of premature senescence. Mechanistically, RV-induced premature senescence correlates with increased DNA double strand breaks (DSBs) and reactive oxygen species (ROS) production in lung cancer cells. Inhibition of ROS production by N-acetylcysteine (NAC) attenuates RV-induced DNA DSBs and premature senescence. Furthermore, we show that RV treatment markedly induces NAPDH oxidase-5 (Nox5) expression in both A549 and H460 cells, suggesting that RV may increase ROS generation in lung cancer cells through upregulating Nox5 expression. Together, these findings demonstrate that low dose RV treatment inhibits lung cancer cell growth via a previously unappreciated mechanism, namely the induction of premature senescence through ROS-mediated DNA damage.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Acetylcysteine / Anticancer properties / Apoptosis / Biology / Blotting, Western / Cancer / Cancer cells / Cancer genetics / Cancer prevention / Cancer therapies / Cancer treatment / Caspase / Caspase-3 / Cell cycle / Cell Line, Tumor / Cellular Senescence - drug effects / Chemotherapy / Deoxyribonucleic acid / DNA / DNA damage / DNA Damage - drug effects / Drug dosages / Fibroblasts / Flow Cytometry / Galactosidase / Grapes / Humans / Ionizing radiation / Laboratories / Lung cancer / Lung diseases / Lung Neoplasms - genetics / Medical prognosis / Medicine / Molecular modelling / NAD(P)H oxidase / Non-small cell lung cancer / Non-small cell lung carcinoma / Oxidative stress / Oxygen / p53 Protein / Pathology / Poly(ADP-ribose) polymerase / Reactive oxygen species / Reactive Oxygen Species - metabolism / Respiratory system agents / Resveratrol / Reverse Transcriptase Polymerase Chain Reaction / Senescence / Stilbenes - pharmacology / Tumor proteins / Wine / Wines / β-Galactosidase