Journal Article

High-Anxious Individuals Show Increased Chronic Stress Burden, Decreased Protective Immunity, and Increased Cancer Progression in a Mouse Model of Squamous Cell Carcinoma

Neri, Eric, Kusewitt, Donna, Saul, Alison N., Holmes, Tyson H., Tillie, Jean M., Dhabhar, Firdaus S., Oberyszyn, Tatiana M., Daugherty, Christine

2012

Overview

In spite of widespread anecdotal and scientific evidence much remains to be understood about the long-suspected connection between psychological factors and susceptibility to cancer. The skin is the most common site of cancer, accounting for nearly half of all cancers in the US, with approximately 2-3 million cases of non-melanoma cancers occurring each year worldwide. We hypothesized that a high-anxious, stress-prone behavioral phenotype would result in a higher chronic stress burden, lower protective-immunity, and increased progression of the immuno-responsive skin cancer, squamous cell carcinoma. SKH1 mice were phenotyped as high- or low-anxious at baseline, and subsequently exposed to ultraviolet-B light (1 minimal erythemal dose (MED), 3 times/week, 10-weeks). The significant strengths of this cancer model are that it uses a normal, immunocompetent, outbred strain, without surgery/injection of exogenous tumor cells/cell lines, and produces lesions that resemble human tumors. Tumors were counted weekly (primary outcome), and tissues collected during early and late phases of tumor development. Chemokine/cytokine gene-expression was quantified by PCR, tumor-infiltrating helper (Th), cytolytic (CTL), and regulatory (Treg) T cells by immunohistochemistry, lymph node T and B cells by flow cytometry, adrenal and plasma corticosterone and tissue vascular-endothelial-growth-factor (VEGF) by ELISA. High-anxious mice showed a higher tumor burden during all phases of tumor development. They also showed: higher corticosterone levels (indicating greater chronic stress burden), increased CCL22 expression and Treg infiltration (increased tumor-recruited immuno-suppression), lower CTACK/CCL27, IL-12, and IFN-γ gene-expression and lower numbers of tumor infiltrating Th and CTLs (suppressed protective immunity), and higher VEGF concentrations (increased tumor angiogenesis/invasion/metastasis). These results suggest that the deleterious effects of high trait anxiety could be: exacerbated by life-stressors, accentuated by the stress of cancer diagnosis/treatment, and mediate increased tumor progression and/or metastasis. Therefore, it may be beneficial to investigate the use of chemotherapy-compatible anxiolytic treatments immediately following cancer diagnosis, and during cancer treatment/survivorship.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Analysis / Angiogenesis / Animal tissues / Animals / Anxiety / Anxiety - complications / Anxiety - immunology / B cells / Behavioral sciences / Biology / Biomedical research / Brain research / Cancer / Cancer metastasis / Carcinoma, Squamous Cell - etiology / Carcinoma, Squamous Cell - psychology / CCL22 protein / Chemokines - metabolism / Chemotherapy / Corticosterone / Corticosterone - blood / Cytometry / Cytotoxicity / Diagnosis / Disease Progression / Disease susceptibility / Enzyme-Linked Immunosorbent Assay / Flow Cytometry / Gene expression / Gene Expression Regulation - immunology / Generalized linear models / Genes / Genotype & phenotype / Immunity / Immunohistochemistry / Immunology / Infiltration / Interferon / Interleukin 12 / Lesions / Lymph nodes / Lymphocytes / Lymphocytes B / Lymphocytes T / Medicine / Melanoma / Metastases / Mice / Mortality / Personality / Physiology / Polymerase Chain Reaction / Psychiatry / Psychological factors / Skin / Skin cancer / Skin diseases / Skin Neoplasms - etiology / Skin Neoplasms - psychology / Social and Behavioral Sciences / Squamous cell carcinoma / Stress / Stress (Psychology) / Stress, Psychological - complications / Stress, Psychological - immunology / Stresses / Studies / Surgery / Survival / T cells / T-Lymphocyte Subsets - immunology / Thorium / Tumor cell lines / Tumor cells / Tumors / Ultraviolet radiation / Ultraviolet Rays / Vascular endothelial growth factor / Vascular Endothelial Growth Factor A - blood / γ-Interferon