Journal Article

mus-52 disruption and metabolic regulation in Neurospora crassa: Transcriptional responses to extracellular phosphate availability

Rossi, Antonio, Martinez-Rossi, Nilce M., Gomes, Eriston V., Pedersoli, Wellington R., Martins, Maíra P., Sanches, Pablo R.

2018

Overview

Advances in the understanding of molecular systems depend on specific tools like the disruption of genes to produce strains with the desired characteristics. The disruption of any mutagen sensitive (mus) genes in the model fungus Neurospora crassa, i.e. mus-51, mus-52, or mus-53, orthologous to the human genes KU70, KU80, and LIG4, respectively, provides efficient tools for gene targeting. Accordingly, we used RNA-sequencing and reverse transcription-quantitative polymerase chain reaction amplification techniques to evaluate the effects of mus-52 deletion in N. crassa gene transcriptional modulation, and thus, infer its influence regarding metabolic response to extracellular availability of inorganic phosphate (Pi). Notably, the absence of MUS-52 affected the transcription of a vast number of genes, highlighting the expression of those coding for transcription factors, kinases, circadian clocks, oxi-reduction balance, and membrane- and nucleolus-related proteins. These findings may provide insights toward the KU molecular mechanisms, which have been related to telomere maintenance, apoptosis, DNA replication, and gene transcription regulation, as well as associated human conditions including immune system disorders, cancer, and aging.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Aging / Analysis / Apoptosis / Bioinformatics / Biological clocks / Biology and Life Sciences / Cancer / Circadian rhythm / Circadian rhythms / Coding / Computational Biology - methods / Deoxyribonucleic acid / Disruption / DNA / DNA binding proteins / DNA biosynthesis / DNA repair / DNA replication / Energy Metabolism - genetics / Enzymes / Extracellular Space - metabolism / Fungal Proteins - genetics / Fungal Proteins - metabolism / Fungi / Gene deletion / Gene expression / Gene Expression Profiling / Gene Ontology / Gene regulation / Gene sequencing / Gene targeting / Genes / Genetics / Genomes / Genomics / Humans / Immune system / Kinases / Life assessment / LIG4 protein / Medical schools / Metabolic response / Metabolism / Methods / Molecular modelling / Molecular Sequence Annotation / Mutation / Neural coding / Neurospora / Neurospora crassa / Neurospora crassa - genetics / Neurospora crassa - metabolism / Nucleoli / Ontology / Phosphates / Phosphates - metabolism / Physical Sciences / Physiology / Polymerase chain reaction / Proteins / Reproducibility of Results / Research and Analysis Methods / Reverse transcription / Ribonucleic acid / RNA / Transcription (Genetics) / Transcription factors / Transcription, Genetic