Journal Article

Characterization of microRNAs of Beta macrocarpa and their responses to Beet necrotic yellow vein virus infection

Liu, Jun-Ying, Yu, Jia-Lin, Wang, Ying, Han, Cheng-Gui, Fan, Hui-Yan, Li, Da-Wei, Zhang, Yong-Liang

2017

Overview

Plant microRNAs (miRNAs) are a class of non-coding RNAs that play important roles in plant development, defense, and symptom development. Here, 547 known miRNAs representing 129 miRNA families, and 282 potential novel miRNAs were identified in Beta macrocarpa using small RNA deep sequencing. A phylogenetic analysis was performed, and 8 Beta lineage-specific miRNAs were identified. Through a differential expression analysis, miRNAs associated with Beet necrotic yellow vein virus (BNYVV) infection were identified and confirmed using a microarray analysis and stem-loop RT-qPCR. In total, 103 known miRNAs representing 38 miRNA families, and 45 potential novel miRNAs were differentially regulated, with at least a two-fold change, in BNYVV-infected plants compared with that of the mock-inoculated control. Targets of these differentially expressed miRNAs were also predicted by degradome sequencing. These differentially expressed miRNAs were involved in hormone biosynthesis and signal transduction pathways, and enhanced axillary bud development and plant defenses. This work is the first to describe miRNAs of the plant genus Beta and may offer a reference for miRNA research in other species in the genus. It provides valuable information on the pathogenicity mechanisms of BNYVV.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Agriculture / Analysis / Arabidopsis / Arabidopsis thaliana / Beta macrocarpa / Beta vulgaris / Beta vulgaris - cytology / Beta vulgaris - genetics / Beta vulgaris - metabolism / Beta vulgaris - virology / Biology / Biology and life sciences / Biosynthesis / Biotechnology / Computer and Information Sciences / DNA methylation / Flowers & plants / Gene expression / Gene Expression Regulation, Plant / Gene sequencing / Genetic aspects / Genomes / Genomics / Health aspects / High-Throughput Nucleotide Sequencing / Infections / Laboratories / Macrocephaly / MicroRNA / MicroRNAs / MicroRNAs - genetics / miRNA / Oryza / Pathogenicity / Pathogens / Phase transitions / Phylogeny / Physiological aspects / Plant Diseases - virology / Plant Growth Regulators - biosynthesis / Plant Leaves - virology / Plant pathology / Plant resistance / Plant virus diseases / Plant viruses / Plant Viruses - physiology / Plants (botany) / Predictive control / Research and analysis methods / Ribonucleic acid / Rice / Risk factors / RNA / RNA polymerase / Sequence Analysis, RNA / Signal Transduction / Studies / Transcription factors / Transduction / Veins (plant anatomy) / Viral infections / Virus diseases / Viruses