Journal Article

Influence of a Non-Hospital Medical Care Facility on Antimicrobial Resistance in Wastewater

Jonas, Daniel, Schilhabel, Markus B., Bäumlisberger, Mathias, Youssar, Loubna

2015

Overview

The global widespread use of antimicrobials and accompanying increase in resistant bacterial strains is of major public health concern. Wastewater systems and wastewater treatment plants are considered a niche for antibiotic resistance genes (ARGs), with diverse microbial communities facilitating ARG transfer via mobile genetic element (MGE). In contrast to hospital sewage, wastewater from other health care facilities is still poorly investigated. At the instance of a nursing home located in south-west Germany, in the present study, shotgun metagenomics was used to investigate the impact on wastewater of samples collected up- and down-stream in different seasons. Microbial composition, ARGs and MGEs were analyzed using different annotation approaches with various databases, including Antibiotic Resistance Ontologies (ARO), integrons and plasmids. Our analysis identified seasonal differences in microbial communities and abundance of ARG and MGE between samples from different seasons. However, no obvious differences were detected between up- and downstream samples. The results suggest that, in contrast to hospitals, sewage from the nursing home does not have a major impact on ARG or MGE in wastewater, presumably due to much less intense antimicrobial usage. Possible limitations of metagenomic studies using high-throughput sequencing for detection of genes that seemingly confer antibiotic resistance are discussed.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Annotations / Annual variations / Antibiotic resistance / Antibiotics / Antiinfectives and antibacterials / Antimicrobial agents / Antimicrobial resistance / Bacteria / Bacteria - classification / Bacteria - drug effects / Bacteria - genetics / Communities / Controlled vocabularies / Deoxyribonucleic acid / Disease control / DNA / Drug resistance / Drug Resistance, Microbial - genetics / Environmental health / Gene sequencing / Genes / Genetic Variation / Genomics / Health care / Health Facilities / Health sciences / Hospital wastes / Hospitals / International economic relations / Interspersed Repetitive Sequences / Medical wastes / Metagenomics / Microbial activity / Microbial drug resistance / Microorganisms / Molecular biology / Mutation / Next-generation sequencing / Nurses / Nursing Homes / Plasmids / Public health / Seasons / Sequence Analysis / Sewage / Sewer systems / Staphylococcus aureus / Staphylococcus infections / Taxonomy / Waste Water - microbiology / Wastewater / Wastewater treatment / Wastewater treatment plants