Journal Article

Prevalence and distribution of Plasmodium vivax Duffy Binding Protein gene duplications in Sudan

Gamil, Eiman, Elfaki, Tarig Mohamed, Ford, Anthony, Abukashawa, Sumaia Mohamed Ahmed, Lo, Eugenia, Ahmed, Safaa, Pestana, Kareen, Elamin, Arwa F., Hamad, Samuel Omer, Abdel Hamid, Muzamil M., Elfaki, Mohammed

2023

Overview

Plasmodium vivax Duffy Binding Protein (PvDBP) is essential for interacting with Duffy antigen receptor for chemokines (DARC) on the surface of red blood cells to allow invasion. Earlier whole genome sequence analyses provided evidence for the duplications of PvDBP . It is unclear whether PvDBP duplications play a role in recent increase of P . vivax in Sudan and in Duffy-negative individuals. In this study, the prevalence and type of PvDBP duplications, and its relationship to demographic and clinical features were investigated. A total of 200 malaria-suspected blood samples were collected from health facilities in Khartoum, River Nile, and Al-Obied. Among them, 145 were confirmed to be P . vivax , and 43 (29.7%) had more than one PvDBP copies with up to four copies being detected. Both the Malagasy and Cambodian types of PvDBP duplication were detected. No significant difference was observed between the two types of duplications between Duffy groups. Parasitemia was significantly higher in samples with the Malagasy-type than those without duplications. No significant difference was observed in PvDBP duplication prevalence and copy number among study sites. The functional significance of PvDBP duplications, especially those Malagasy-type that associated with higher parasitemia, merit further investigations.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Analysis / Antigens / Antigens, Protozoan / Biology and Life Sciences / Blood groups / Cell surface / Chemokines / Copy number / Diagnosis / Distribution / Duffy antigen / Duffy Blood-Group System - genetics / Duffy Blood-Group System - metabolism / Erythrocytes / Erythrocytes - metabolism / Gene Duplication / Gene mutations / Genomes / Health aspects / Health care facilities / Humans / Infections / Malaria / Malaria, Vivax - epidemiology / Malaria, Vivax - genetics / Medicine and Health Sciences / Nucleotide sequence / Parasitemia / Parasitemia - genetics / Parasites / People and Places / Plasmodium vivax / Prevalence / Proteins / Protozoa / Protozoan Proteins - metabolism / Public health / Research and Analysis Methods / Sudan - epidemiology / Vector-borne diseases