Journal Article

Towards the Human Colorectal Cancer Microbiome

Boleij, Annemarie, Roelofs, Rian, Hall, Neil, Tjalsma, Harold, Dutilh, Bas E., Marchesi, Julian R., Peters, Wilbert H. M.

2011

Overview

Multiple factors drive the progression from healthy mucosa towards sporadic colorectal carcinomas and accumulating evidence associates intestinal bacteria with disease initiation and progression. Therefore, the aim of this study was to provide a first high-resolution map of colonic dysbiosis that is associated with human colorectal cancer (CRC). To this purpose, the microbiomes colonizing colon tumor tissue and adjacent non-malignant mucosa were compared by deep rRNA sequencing. The results revealed striking differences in microbial colonization patterns between these two sites. Although inter-individual colonization in CRC patients was variable, tumors consistently formed a niche for Coriobacteria and other proposed probiotic bacterial species, while potentially pathogenic Enterobacteria were underrepresented in tumor tissue. As the intestinal microbiota is generally stable during adult life, these findings suggest that CRC-associated physiological and metabolic changes recruit tumor-foraging commensal-like bacteria. These microbes thus have an apparent competitive advantage in the tumor microenvironment and thereby seem to replace pathogenic bacteria that may be implicated in CRC etiology. This first glimpse of the CRC microbiome provides an important step towards full understanding of the dynamic interplay between intestinal microbial ecology and sporadic CRC, which may provide important leads towards novel microbiome-related diagnostic tools and therapeutic interventions.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Aged / Apoptosis / Bacteria / Bacterial Adhesion / Bacterial infections / Biology / Biopsy / Breast cancer / Cancer / Colon / Colonization / Colorectal cancer / Colorectal carcinoma / Colorectal Neoplasms - microbiology / Colorectal Neoplasms - pathology / Cytochrome / Denaturing Gradient Gel Electrophoresis / Deoxyribonucleic acid / Development and progression / Diagnostic software / Diagnostic systems / DNA / DNA Fingerprinting / DNA, Intergenic - genetics / Dysbacteriosis / Ecology / Etiology / Faecalibacterium / Female / Forage / Forages / Gastrointestinal diseases / Growth factors / Helicobacter pylori / Humans / Infections / Inflammation / Inflammatory bowel disease / Intestinal microflora / Intestinal Mucosa - microbiology / Intestinal Mucosa - pathology / Intestine / Laboratories / Male / Medical research / Medicine / Metagenome / Microbiota (Symbiotic organisms) / Microorganisms / Middle Aged / Mucosa / Oncology / Patients / Probiotics / Recruitment / Ribosomes - genetics / RNA / RNA, Bacterial - genetics / RNA, Ribosomal, 16S - genetics / rRNA / Sequence Analysis, RNA / Therapeutic applications / Trends / Tumorigenesis / Tumors