Journal Article

Guidelines for morpholino use in zebrafish

Meng, Anming, Yelon, Deborah, Mochizuki, Naoki, Moens, Cecilia B., Lawson, Nathan D., Ekker, Stephen C., Weinstein, Brant M., Stainier, Didier Y. R., Raz, Erez, Schulte-Merker, Stefan, Panula, Pertti, Wilson, Stephen W., Ingham, Philip W., Ramakrishnan, Lalita, Burdine, Rebecca D., Amacher, Sharon L., Mullins, Mary C., Neuhauss, Stephan C. F., Eisen, Judith S.

2017

Overview

About the Authors: Didier Y. R. Stainier * E-mail: [e-mail] (DYRS); [e-mail] (CBM) Affiliation: Department of Developmental Genetics, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany ORCID http://orcid.org/0000-0002-0382-0026 Erez Raz Affiliation: Institute of Cell Biology, ZBME, University of Münster, Münster, Germany ORCID http://orcid.org/0000-0002-6347-3302 Nathan D. Lawson Affiliation: Department of Molecular, Cell, and Cancer Biology, University of Massachusetts Medical School, Worcester, Massachusetts, United States of America Stephen C. Ekker Affiliation: Mayo Clinic, Rochester, Minnesota, United States of America Rebecca D. Burdine Affiliation: Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America Judith S. Eisen Affiliation: Institute of Neuroscience, University of Oregon, Eugene, Oregon, United States of America ORCID http://orcid.org/0000-0003-1229-1696 Philip W. Ingham Affiliations Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, The Living Systems Institute, University of Exeter, Exeter, United Kingdom Stefan Schulte-Merker Affiliation: Institute of Cardiovascular Organogenesis and Regeneration, WWU Münster, Faculty of Medicine, Münster, Germany Deborah Yelon Affiliation: Division of Biological Sciences, University of California, San Diego, La Jolla, California, United States of America Brant M. Weinstein Affiliation: Division of Developmental Biology, NICHD, NIH, Bethesda, Maryland, United States of America Mary C. Mullins Affiliation: Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, United States of America ORCID http://orcid.org/0000-0002-9979-1564 Stephen W. Wilson Affiliation: Department of Cell and Developmental Biology, University College London, London, United Kingdom ORCID http://orcid.org/0000-0002-8557-5940 Lalita Ramakrishnan Affiliation: Molecular Immunity Unit, Department of Medicine, University of Cambridge, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom Sharon L. Amacher Affiliation: Departments of Molecular Genetics and Biological Chemistry and Pharmacology, Ohio State University, Columbus, Ohio, United States of America Stephan C. F. Neuhauss Affiliation: Institute of Molecular Life Sciences, University of Zurich, Zurich, Switzerland ORCID http://orcid.org/0000-0002-9615-480X Anming Meng Affiliation: School of Life Sciences, Tsinghua University, Beijing, China Naoki Mochizuki Affiliation: National Cerebral and Cardiovascular Center Research Institute, Osaka, Japan ORCID http://orcid.org/0000-0002-3938-9602 Pertti Panula Affiliation: Department of Anatomy and Neuroscience Center, University of Helsinki, Helsinki, Finland Cecilia B. Moens * E-mail: [e-mail] (DYRS); [e-mail] (CBM) Affiliation: Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of AmericaCitation: Stainier DYR, Raz E, Lawson ND, Ekker SC, Burdine RD, Eisen JS, et al. Additionally, mutant alleles for many genes are now readily available through zebrafish community resource centers. [...]MOs should be used alongside mutant(s) for the corresponding gene. [...]a word of caution that previous publication of MOs is not a guarantee of their fidelity, particularly if a new phenotype is being described. [...]we hope that these brief and mostly conceptual guidelines will assist scientists working with zebrafish as well as those assessing manuscripts and grant proposals based on experiments using zebrafish.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Animals / Biology and Life Sciences / Danio rerio / Developmental biology / Drug dosages / Embryos / Experiments / Female / Genes / Genetic aspects / Genetic engineering / Genetic Techniques - standards / Genomes / Life sciences / Male / Medical research / Medicine / Methods / Molecular biology / Morpholinos - adverse effects / Morpholinos - genetics / Mutation / Neurosciences / Oligomers / Research and Analysis Methods / Studies / Viewpoints / Xenopus / Zebrafish / Zebrafish - genetics