Journal Article

wnt16 regulates spine and muscle morphogenesis through parallel signals from notochord and dermomyotome

Gómez, Arianna Ericka, Rojas, Maria F., Maves, Lisa, Swearer, Avery Angell, Sethuraman, Visali, Gardiner, Edith M., Callies, Lulu K., Rai, Jyoti, Hsu, Yi-Hsiang, Watson, Claire J., Addish, Sumaya, Morfin Montes de Oca, Ernesto, Kwon, Ronald Young, Busse, Bjorn, Karasik, David, Ahmed, Ali R., Cronrath, Andrea R., Farr, Gist H., Monstad-Rios, Adrian T., Tang, W. Joyce, Fiedler, Imke A. K.

2022

Overview

Bone and muscle are coupled through developmental, mechanical, paracrine, and autocrine signals. Genetic variants at the CPED1-WNT16 locus are dually associated with bone- and muscle-related traits. While Wnt16 is necessary for bone mass and strength, this fails to explain pleiotropy at this locus. Here, we show wnt16 is required for spine and muscle morphogenesis in zebrafish. In embryos, wnt16 is expressed in dermomyotome and developing notochord, and contributes to larval myotome morphology and notochord elongation. Later, wnt16 is expressed at the ventral midline of the notochord sheath, and contributes to spine mineralization and osteoblast recruitment. Morphological changes in wnt16 mutant larvae are mirrored in adults, indicating that wnt16 impacts bone and muscle morphology throughout the lifespan. Finally, we show that wnt16 is a gene of major effect on lean mass at the CPED1-WNT16 locus. Our findings indicate that Wnt16 is secreted in structures adjacent to developing bone (notochord) and muscle (dermomyotome) where it affects the morphogenesis of each tissue, thereby rendering wnt16 expression into dual effects on bone and muscle morphology. This work expands our understanding of wnt16 in musculoskeletal development and supports the potential for variants to act through WNT16 to influence bone and muscle via parallel morphogenetic processes.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Animals / Annotations / Autocrine signalling / Biology and Life Sciences / Bone density / Bone mass / Cell cycle / Cellular signal transduction / Embryos / Fractures / Gene expression / Genetic aspects / Genetic diversity / Genetic variance / Genetic variation / Larva / Lead / Life span / Medicine and Health Sciences / Mineralization / Morphogenesis / Morphogenesis - genetics / Morphology / Muscles / Musculoskeletal system / Notochord / Paracrine signalling / Physiological aspects / Physiological research / Pleiotropy / Research and Analysis Methods / Sarcopenia / Spine / Wnt proteins / Wnt Proteins - genetics / Zebrafish - genetics / Zebrafish Proteins - genetics