Journal Article

Recognition of specific sialoglycan structures by oral streptococci impacts the severity of endocardial infection

Barnard, Karen N., Yakovenko, Olga, Parrish, Colin R., Xiong, Yan, Li, Liang, Iverson, T. M., Wong, Maurice, Bensing, Barbara A., Thomas, Wendy E., Lebrilla, Carlito B., Sullam, Paul M.

2019

Overview

Streptococcus gordonii and Streptococcus sanguinis are primary colonizers of the tooth surface. Although generally non-pathogenic in the oral environment, they are a frequent cause of infective endocarditis. Both streptococcal species express a serine-rich repeat surface adhesin that mediates attachment to sialylated glycans on mucin-like glycoproteins, but the specific sialoglycan structures recognized can vary from strain to strain. Previous studies have shown that sialoglycan binding is clearly important for aortic valve infections caused by some S. gordonii, but this process did not contribute to the virulence of a strain of S. sanguinis. However, these streptococci can bind to different subsets of sialoglycan structures. Here we generated isogenic strains of S. gordonii that differ only in the type and range of sialoglycan structures to which they adhere and examined whether this rendered them more or less virulent in a rat model of endocarditis. The findings indicate that the recognition of specific sialoglycans can either enhance or diminish pathogenicity. Binding to sialyllactosamine reduces the initial colonization of mechanically-damaged aortic valves, whereas binding to the closely-related trisaccharide sialyl T-antigen promotes higher bacterial densities in valve tissue 72 hours later. A surprising finding was that the initial attachment of streptococci to aortic valves was inversely proportional to the affinity of each strain for platelets, suggesting that binding to platelets circulating in the blood may divert bacteria away from the endocardial surface. Importantly, we found that human and rat platelet GPIbα (the major receptor for S. gordonii and S. sanguinis on platelets) display similar O-glycan structures, comprised mainly of a di-sialylated core 2 hexasaccharide, although the rat GPIbα has a more heterogenous composition of modified sialic acids. The combined results suggest that streptococcal interaction with a minor O-glycan on GPIbα may be more important than the over-all affinity for GPIbα for pathogenic effects.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Affinity / Animals / Antigens / Aortic valve / Bacteria / Binding / Bioengineering / Biology and Life Sciences / Biomedical research / Blood circulation / Colonization / Disease Models, Animal / Endocarditis / Endocarditis, Bacterial - immunology / Endocarditis, Bacterial - pathology / Female / Funding / Genomes / Glycan / Glycoproteins / Glycoproteins - immunology / Gram-positive bacteria / Heart valve diseases / Heart valves / Humans / Immunology / Infection / Infective endocarditis / Laboratory rats / Ligands / Male / Medicine and Health Sciences / Microbiota (Symbiotic organisms) / Mucin / Oral cavity / Oral infection / Pathogenesis / Pathogenicity / Pathogens / Physical Sciences / Platelets / Polypeptides / Polysaccharides / Proteins / Rats / Rats as laboratory animals / Rats, Sprague-Dawley / Recognition / Risk factors / Serine / Severity of Illness Index / Sialic acids / Sialic Acids - immunology / Streptococcal Infections - immunology / Streptococcal Infections - pathology / Streptococcus gordonii / Streptococcus gordonii - immunology / Streptococcus gordonii - pathogenicity / Streptococcus infections / Streptococcus sanguinis / Streptococcus sanguis - immunology / Streptococcus sanguis - pathogenicity / Teeth / Veterinary colleges / Veterinary medicine / Virulence / Virulence (Microbiology)