Journal Article

Global reference mapping of human transcription factor footprints

Kaul, Rajinder, Neri, Fidencio, Halow, Jessica, Stamatoyannopoulos, John A., Bates, Daniel, Buckley, Michael, Rynes, Eric, Diegel, Morgan, Nelson, Jemma, Reynolds, Alex, Dunn, Douglas, Frerker, Mark, Vierstra, Jeff, Meuleman, Wouter, Sandstrom, Richard, Haugen, Eric, Johnson, Audra, Lazar, John, Lee, Kristen

2020

Overview

Combinatorial binding of transcription factors to regulatory DNA underpins gene regulation in all organisms. Genetic variation in regulatory regions has been connected with diseases and diverse phenotypic traits 1 , but it remains challenging to distinguish variants that affect regulatory function 2 . Genomic DNase I footprinting enables the quantitative, nucleotide-resolution delineation of sites of transcription factor occupancy within native chromatin 3 – 6 . However, only a small fraction of such sites have been precisely resolved on the human genome sequence 6 . Here, to enable comprehensive mapping of transcription factor footprints, we produced high-density DNase I cleavage maps from 243 human cell and tissue types and states and integrated these data to delineate about 4.5 million compact genomic elements that encode transcription factor occupancy at nucleotide resolution. We map the fine-scale structure within about 1.6 million DNase I-hypersensitive sites and show that the overwhelming majority are populated by well-spaced sites of single transcription factor–DNA interaction. Cell-context-dependent cis -regulation is chiefly executed by wholesale modulation of accessibility at regulatory DNA rather than by differential transcription factor occupancy within accessible elements. We also show that the enrichment of genetic variants associated with diseases or phenotypic traits in regulatory regions 1 , 7 is almost entirely attributable to variants within footprints, and that functional variants that affect transcription factor occupancy are nearly evenly partitioned between loss- and gain-of-function alleles. Unexpectedly, we find increased density of human genetic variation within transcription factor footprints, revealing an unappreciated driver of cis -regulatory evolution. Our results provide a framework for both global and nucleotide-precision analyses of gene regulatory mechanisms and functional genetic variation. A high-density DNase I cleavage map from 243 human cell and tissue types provides a genome-wide, nucleotide-resolution map of human transcription factor footprints.

• Publisher: Nature Publishing Group UK,Nature Publishing Group
• Subject: 38/22 / 631/208/177 / 631/208/191 / 631/208/200 / 631/208/212/177 / Accessibility / Analysis / Binding sites (Biochemistry) / Chromosome mapping / Combinatorial analysis / Consensus Sequence / Datasets / Density / Deoxyribonuclease / Deoxyribonuclease I - metabolism / Deoxyribonucleic acid / DNA / DNA - genetics / DNA - metabolism / DNA Footprinting - standards / Footprinting / Footprints / Gene mapping / Gene regulation / Genetic diversity / Genetic variance / Genetics, Population / Genome, Human - genetics / Genome-wide association studies / Genome-Wide Association Study / Genomes / Human chromosomes / Humanities and Social Sciences / Humans / Identification and classification / Mapping / Methods / Models, Molecular / multidisciplinary / Nucleotides / Occupancy / Polymorphism, Single Nucleotide / Regulatory mechanisms (biology) / Regulatory sequences / Regulatory Sequences, Nucleic Acid - genetics / Science / Science (multidisciplinary) / Transcription factors / Transcription Factors - metabolism