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Rapid evaluation of 25 key sphingolipids and phosphosphingolipids in human plasma by LC-MS/MS
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Rapid evaluation of 25 key sphingolipids and phosphosphingolipids in human plasma by LC-MS/MS
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Journal Article
Rapid evaluation of 25 key sphingolipids and phosphosphingolipids in human plasma by LC-MS/MS
2015
Overview
We report on a new, sensitive, and fast LC-MS/MS method for the simultaneous determination of 25 key sphingolipid components in human plasma, including phosphorylated sphinganine and sphingosine, in a single 9-min run. This method enables an effective and high-throughput coverage of the metabolic changes involving the sphingolipidome during physiological or pathological states. The method is based on liquid–liquid extraction followed by reversed-phase LC-MS/MS. Exogenous odd-chain lipids are used as cost-effective but reliable internal standards. The method was fully validated in surrogate matrix and naive human plasma following FDA guidelines. Sample stability and dilution integrity were also tested and verified.
Publisher
Springer Berlin Heidelberg,Springer,Springer Nature B.V
Subject
/ Characterization and Evaluation of Materials
/ Chemistry and Materials Science
/ Chromatography, High Pressure Liquid - economics
/ Chromatography, High Pressure Liquid - methods
/ Chromatography, Reverse-Phase - economics
/ Chromatography, Reverse-Phase - methods
/ Female
/ Human
/ Humans
/ Identification and classification
/ Lipids
/ Liquid-Liquid Extraction - economics
/ Liquid-Liquid Extraction - methods
/ Male
/ Methods
/ Monitoring/Environmental Analysis
/ Plasma
