Journal Article

Drosophila-associated bacteria differentially shape the nutritional requirements of their host during juvenile growth

Leulier, François, Institut de Génomique Fonctionnelle de Lyon (IGFL) ; École normale supérieure de Lyon (ENS de Lyon) ; Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Biologie Fonctionnelle, Insectes et Interactions (BF2I) ; Institut National des Sciences Appliquées de Lyon (INSA Lyon) ; Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Parisot, Nicolas, Equipe de recherche européenne en algorithmique et biologie formelle et expérimentale (ERABLE) ; Centre Inria de l'Université Grenoble Alpes ; Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Aut

2020

Overview

The interplay between nutrition and the microbial communities colonizing the gastrointestinal tract (i.e., gut microbiota) determines juvenile growth trajectory. Nutritional deficiencies trigger developmental delays, and an immature gut microbiota is a hallmark of pathologies related to childhood undernutrition. However, how host-associated bacteria modulate the impact of nutrition on juvenile growth remains elusive. Here, using gnotobiotic Drosophila melanogaster larvae independently associated with Acetobacter pomorum WJL (Ap WJL) and Lactobacillus plantarum NC8 (Lp NC8), 2 model Drosophila-associated bacteria, we performed a large-scale, systematic nutritional screen based on larval growth in 40 different and precisely controlled nutritional environments. We combined these results with genome-based metabolic network reconstruction to define the biosynthetic capacities of Drosophila germ-free (GF) larvae and its 2 bacterial partners. We first established that Ap WJL and Lp NC8 differentially fulfill the nutritional requirements of the ex-GF larvae and parsed such difference down to individual amino acids, vitamins, other micronutrients, and trace metals. We found that Drosophila-associated bacteria not only fortify the host's diet with essential nutrients but, in specific instances, functionally compensate for host auxotrophies by either providing a metabolic intermediate or nutrient derivative to the host or by uptaking, concentrating, and delivering contaminant traces of micronutrients. Our systematic work reveals that beyond the molecular dialogue engaged between the host and its bacterial partners, Drosophila and its associated bacteria establish an integrated nutritional network relying on nutrient provision and utilization.

• Publisher: CCSD,Public Library of Science,Public Library of Science (PLoS)
• Subject: Amino acids / Analysis / Bacteria / Biology and Life Sciences / Children / Contaminants / Diet / Digestive system / Digestive tract / Drosophila / Ecology, environment / Essential nutrients / Gastrointestinal system / Gastrointestinal tract / Genomes / Genomics / Germfree / Gnotobiotic / Growth / Insects / Instrument industry / Intestinal microflora / Larvae / Larval development / Life Sciences / Malnutrition / Medicine and Health Sciences / Metabolic networks / Microbial activity / Microbiota / Microbiota (Symbiotic organisms) / Micronutrients / Microorganisms / Nutrient deficiency / Nutrient utilization / Nutrients / Nutrition / Nutrition research / Nutritional requirements / Physical Sciences / Physiology / Requirements / Research and Analysis Methods / Software / Symbiosis / Trace metals / Twins / Undernutrition / Vitamins
• ISBN: 9780005229408, 0005229405