Journal Article

Global emergence and population dynamics of divergent serotype 3 CC180 pneumococci

Hanage, William P., von Gottberg, Anne, Gladstone, Rebecca A., Kwambana-Adams, Brenda A., Azarian, Taj, Breiman, Robert F., Reid, Raymond, du Plessis, Mignon, Ho, PL, O’Brien, Katherine L., Clarke, Stuart C., Bentley, Stephen D., Everett, Dean, Thompson, Claudette M., Metcalf, Benjamin J., Cornick, Jennifer, Georgieva, Maria, Skoczynska, Anna, Sadowy, Ewa, Hryniewicz, Waleria, Pollard, Andrew J., Mitchell, Patrick K., Moïsi, Jennifer C., McGee, Lesley, Ghouila, Amel, Antonio, Martin, Beall, Bernard

2018

Overview

Streptococcus pneumoniae serotype 3 remains a significant cause of morbidity and mortality worldwide, despite inclusion in the 13-valent pneumococcal conjugate vaccine (PCV13). Serotype 3 increased in carriage since the implementation of PCV13 in the USA, while invasive disease rates remain unchanged. We investigated the persistence of serotype 3 in carriage and disease, through genomic analyses of a global sample of 301 serotype 3 isolates of the Netherlands3-31 (PMEN31) clone CC180, combined with associated patient data and PCV utilization among countries of isolate collection. We assessed phenotypic variation between dominant clades in capsule charge (zeta potential), capsular polysaccharide shedding, and susceptibility to opsonophagocytic killing, which have previously been associated with carriage duration, invasiveness, and vaccine escape. We identified a recent shift in the CC180 population attributed to a lineage termed Clade II, which was estimated by Bayesian coalescent analysis to have first appeared in 1968 [95% HPD: 1939-1989] and increased in prevalence and effective population size thereafter. Clade II isolates are divergent from the pre-PCV13 serotype 3 population in non-capsular antigenic composition, competence, and antibiotic susceptibility, the last of which resulting from the acquisition of a Tn916-like conjugative transposon. Differences in recombination rates among clades correlated with variations in the ATP-binding subunit of Clp protease, as well as amino acid substitutions in the comCDE operon. Opsonophagocytic killing assays elucidated the low observed efficacy of PCV13 against serotype 3. Variation in PCV13 use among sampled countries was not independently correlated with the CC180 population shift; therefore, genotypic and phenotypic differences in protein antigens and, in particular, antibiotic resistance may have contributed to the increase of Clade II. Our analysis emphasizes the need for routine, representative sampling of isolates from disperse geographic regions, including historically under-sampled areas. We also highlight the value of genomics in resolving antigenic and epidemiological variations within a serotype, which may have implications for future vaccine development.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Amino acids / Antibiotic resistance / Antibiotics / Antigens / Bayes Theorem / Bayesian analysis / Biology and Life Sciences / Biomedical research / Capsular polysaccharides / Carrier State - epidemiology / Chromosomes / Computer and Information Sciences / Disease control / Disease prevention / Drug resistance in microorganisms / Earth Sciences / Ecology and Environmental Sciences / Epidemiology / Evolution, Molecular / Genetics, Population - methods / Genomes / Genomic analysis / Genomics / Health surveillance / Historical account / Hospitals / Humans / Invasiveness / Laboratories / Life Sciences / Medical research / Medicine and Health Sciences / Meningitis / Microbial drug resistance / Morbidity / Mortality / Murder / Phenotypic variations / Phylogeny / Pneumococcal Infections - immunology / Pneumococcal Infections - transmission / Pneumococcal Vaccines - immunology / Pneumonia / Polysaccharides / Population / Population biology / Population Dynamics / Population number / Prevalence / Proteases / Public health / Recombination / Respiratory diseases / Serogroup / Serotyping - methods / Software / Streptococcus infections / Streptococcus pneumoniae / Streptococcus pneumoniae - genetics / Streptococcus pneumoniae - immunology / Streptococcus pneumoniae - pathogenicity / Transposons / Vaccine development / Vaccines / Vaccines, Conjugate / Whole Genome Sequencing - methods / Zeta potential