Journal Article

Stress and odorant receptor feedback during a critical period after hatching regulates olfactory sensory neuron differentiation in Drosophila

Jafari, Shadi, Henriksson, Johan, Yan, Hua, Alenius, Mattias

2021

Overview

Here, we reveal that the regulation of Drosophila odorant receptor (OR) expression during the pupal stage is permissive and imprecise. We found that directly after hatching an OR feedback mechanism both directs and refines OR expression. We demonstrate that, as in mice, dLsd1 and Su(var)3-9 balance heterochromatin formation to direct OR expression. We show that the expressed OR induces dLsd1 and Su(var)3-9 expression, linking OR level and possibly function to OR expression. OR expression refinement shows a restricted duration, suggesting that a gene regulatory critical period brings olfactory sensory neuron differentiation to an end. Consistent with a change in differentiation, stress during the critical period represses dLsd1 and Su(var)3-9 expression and makes the early permissive OR expression permanent. This induced permissive gene regulatory state makes OR expression resilient to stress later in life. Hence, during a critical period OR feedback, similar to in mouse OR selection, defines adult OR expression in Drosophila .

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Animals / Animals, Genetically Modified / Biology and Life Sciences / Cell differentiation / Cell Differentiation - genetics / Chromatin Assembly and Disassembly - genetics / Critical period / Differentiation / Drosophila / Drosophila - genetics / Drosophila - growth & development / Drosophila - metabolism / Drosophila Proteins - genetics / Drosophila Proteins - metabolism / Drosophila Proteins - physiology / Eclosion / Ectopic expression / Feedback, Physiological - physiology / Female / Fruit flies / Gene expression / Gene Expression Regulation, Developmental / Gene sequencing / Genotype & phenotype / Hatching / Heterochromatin / Heterochromatin - metabolism / Humans / Insects / Male / Mathematical models / Neural networks / Neurogenesis - genetics / Neurogenesis - physiology / Observations / Odorant-binding protein / Odors / Olfactory Bulb - cytology / Olfactory Bulb - growth & development / Olfactory Bulb - metabolism / Olfactory receptor neurons / Olfactory Receptor Neurons - physiology / Olfactory receptors / Oxidoreductases, N-Demethylating - genetics / Oxidoreductases, N-Demethylating - metabolism / Phenotypes / Physical Sciences / Physiological aspects / Plastic properties / Plasticity / Pupa / Receptors / Receptors, Odorant - genetics / Receptors, Odorant - metabolism / Receptors, Odorant - physiology / Regulatory sequences / Repressor Proteins - genetics / Repressor Proteins - metabolism / Research and Analysis Methods / Sensory receptors / Social Sciences / Stress / Stress (Physiology) / Stress, Physiological - physiology / Taste receptors / Time Factors / Vertebrates