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Nanopore adaptive sampling: a tool for enrichment of low abundance species in metagenomic samples
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Nanopore adaptive sampling: a tool for enrichment of low abundance species in metagenomic samples
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Journal Article
Nanopore adaptive sampling: a tool for enrichment of low abundance species in metagenomic samples
2022
Overview
Adaptive sampling is a method of software-controlled enrichment unique to nanopore sequencing platforms. To test its potential for enrichment of rarer species within metagenomic samples, we create a synthetic mock community and construct sequencing libraries with a range of mean read lengths. Enrichment is up to 13.87-fold for the least abundant species in the longest read length library; factoring in reduced yields from rejecting molecules the calculated efficiency raises this to 4.93-fold. Finally, we introduce a mathematical model of enrichment based on molecule length and relative abundance, whose predictions correlate strongly with mock and complex real-world microbial communities.
Publisher
BioMed Central,Springer Nature B.V,BMC
