Journal Article

Basolateral BMP Signaling in Polarized Epithelial Cells

Horiguchi, Kana, Miyazawa, Keiji, Sugiya, Hiroshi, Fukasawa, Akira, Sakamoto, Kei, Saitoh, Masao, Shirakihara, Takuya, Morita, Ikuo, Beppu, Hideyuki, Fujita, Yasuyuki, Miyazono, Kohei

2013

Overview

Bone morphogenetic proteins (BMPs) regulate various biological processes, mostly mediated by cells of mesenchymal origin. However, the roles of BMPs in epithelial cells are poorly understood. Here, we demonstrate that, in polarized epithelial cells, BMP signals are transmitted from BMP receptor complexes exclusively localized at the basolateral surface of the cell membrane. In addition, basolateral stimulation with BMP increased expression of components of tight junctions and enhanced the transepithelial resistance (TER), counteracting reduction of TER by treatment with TGF-β or an anti-tumor drug. We conclude that BMPs maintain epithelial polarity via intracellular signaling from basolaterally localized BMP receptors.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Animals / Biochemistry / Biological activity / Biology / Bone diseases / Bone Morphogenetic Protein 4 - genetics / Bone Morphogenetic Protein 4 - metabolism / Bone Morphogenetic Protein 4 - pharmacology / Bone Morphogenetic Protein 6 - genetics / Bone Morphogenetic Protein 6 - metabolism / Bone Morphogenetic Protein 6 - pharmacology / Bone Morphogenetic Protein Receptors - genetics / Bone Morphogenetic Protein Receptors - metabolism / Bone morphogenetic proteins / Cancer / Cell Line / Cell Polarity - drug effects / Cell signaling / Cell surface / Cisplatin - pharmacology / Cytokines / Dogs / Epithelial cells / Epithelial Cells - cytology / Epithelial Cells - drug effects / Epithelial Cells - metabolism / Gene Expression / Growth Differentiation Factor 2 - genetics / Growth Differentiation Factor 2 - metabolism / Growth Differentiation Factor 2 - pharmacology / Growth factors / Injuries / Interdisciplinary aspects / Intracellular signalling / Kidneys / Kinases / Laboratories / Medicine / Mesenchyme / Mice / Myoblasts - cytology / Myoblasts - drug effects / Myoblasts - metabolism / Pathology / Phosphorylation / Physiology / Polarity / Proteins / Pulmonary hypertension / Receptors / Rodents / Science / Signal Transduction / Tight junctions / Tight Junctions - drug effects / Tight Junctions - genetics / Tight Junctions - metabolism / Transforming Growth Factor beta - pharmacology / Transforming growth factors