Journal Article

Isolation and characterization of lumpy skin disease virus from cattle in India

Barua, Sanjay, Gupta, Madhurendu K., Riyesh, Thachamvally, Chander, Yogesh, Shanmugasundaram, Karuppusamy, Chaudhary, Khushboo, Kumar, Sanjit, Kumar, Naveen, Tripathi, Bhupendra N., Khandelwal, Nitin, Pal, Yash, Kumar, Ram, Kumar, Anand

2021

Overview

Lumpy skin disease (LSD) has devastating economic impact. During the last decade, LSD had spread to climatically new and previously disease-free countries, which also includes its recent emergence in the Indian subcontinent (2019). This study deals with the LSD outbreak(s) from cattle in Ranchi (India). Virus was isolated from the scabs (skin lesions) in the primary goat kidney cells. Phylogenetic analysis based on nucleotide sequencing of LSD virus (LSDV) ORF011, ORF012 and ORF036 suggested that the isolated virus (LSDV/ Bos taurus -tc/India/2019/Ranchi) is closely related to Kenyan LSDV strains. Further, we adapted the isolated virus in Vero cells. Infection of the isolated LSDV to Vero cells did not produce cytopathic effect (CPE) until the 4 th blind passage, but upon adaptation, it produced high viral titres in the cultured cells. The kinetics of viral DNA synthesis and one-step growth curve analysis suggested that Vero cell-adapted LSDV initiates synthesizing its genome at ~24 hours post-infection (hpi) with a peak level at ~96 hpi whereas evidence of progeny virus particles was observed at 36–48 hours (h) with a peak titre at ~120 h. To the best of our knowledge, this study describes the first successful isolation of LSDV in India, besides providing insights into the life cycle Vero cell-adapted LSDV.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Amplification / Animal diseases / Animals / Ankyrins / Biology and Life Sciences / Cattle / Chlorocebus aethiops / Computer and Information Sciences / Computer programs / Deoxyribonucleic acid / Diagnosis / Disease Outbreaks / Diseases / DNA / DNA virus infections / DNA viruses / DNA-directed RNA polymerase / Drafting software / Editing / Electronic mail / G protein-coupled receptors / Genome, Viral / Identification and classification / India - epidemiology / Livestock / Livestock industry / Lumpy skin disease / Lumpy Skin Disease - epidemiology / Lumpy Skin Disease - genetics / Lumpy skin disease virus - genetics / Lumpy skin disease virus - isolation & purification / Lumpy skin disease virus - metabolism / Mail / Nucleotides / Obstetrics / Open Reading Frames / Pathology / People and Places / Phylogenetics / Phylogeny / Physical Sciences / Polymerase chain reaction / Research and Analysis Methods / Research facilities / Reviews / RNA polymerase / Segments / Sequences / Skin / Skin diseases / Software / Vero Cells / Viral diseases / Viruses