Journal Article

Comparative genomic analyses reveal diverse virulence factors and antimicrobial resistance mechanisms in clinical Elizabethkingia meningoseptica strains

Blom, Jochen, Architecture et fonction des macromolécules biologiques (AFMB) ; Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Department of Biostatistics, School of Public Health, University of Michigan, Michigan State University System, Architecture et fonction des Macromolécules Biologiques - UMR 6098 (AFMB) ; Université de Provence - Aix-Marseille 1-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU), Soehnlen, Marty, Chen, Shicheng, Henrissat, Bernard, United States Department of Health & Human Services National Institutes of Health (NIH) - USA R37AI21884, Walker, Edward, D, Id, Jochen, Blom, King Abdulaziz University, Terrapon, Nicolas

2019

Overview

Three human clinical isolates of bacteria (designated strains Em1, Em2 and Em3) had high average nucleotide identity (ANI) to Elizabethkingia meningoseptica. Their genome sizes (3.89, 4.04 and 4.04 Mb) were comparable to those of other Elizabethkingia species and strains, and exhibited open pan-genome characteristics, with two strains being nearly identical and the third divergent. These strains were susceptible only to trimethoprim/sulfamethoxazole and ciprofloxacin amongst 16 antibiotics in minimum inhibitory tests. The resistome exhibited a high diversity of resistance genes, including 5 different lactamase- and 18 efflux protein- encoding genes. Forty-four genes encoding virulence factors were conserved among the strains. Sialic acid transporters and curli synthesis genes were well conserved in E. meningoseptica but absent in E. anophelis and E. miricola. E. meningoseptica carried several genes contributing to biofilm formation. 58 glycoside hydrolases (GH) and 25 putative polysaccharide utilization loci (PULs) were found. The strains carried numerous genes encoding two-component system proteins (56), transcription factor proteins (187~191), and DNA-binding proteins (6~7). Several prophages and CRISPR/Cas elements were uniquely present in the genomes.

• Publisher: HAL CCSD,Public Library of Science,Public Library of Science (PLoS)
• Subject: Anti-Bacterial Agents - therapeutic use / Antibiotics / Antimicrobial agents / Antimicrobial resistance / Bacteria / Binding proteins / Biofilms / Biofilms - growth & development / Biology and Life Sciences / Ciprofloxacin / Clinical isolates / Clustered Regularly Interspaced Short Palindromic Repeats - genetics / Comparative Genomic Hybridization / CRISPR / Deoxyribonucleic acid / DNA / DNA sequencing / DNA-binding protein / DNA-Binding Proteins - genetics / Drug resistance / Drug Resistance, Bacterial - genetics / Efflux / Epidemics / Flavobacteriaceae - genetics / Flavobacteriaceae - pathogenicity / Flavobacteriaceae Infections - drug therapy / Flavobacteriaceae Infections - epidemiology / Flavobacteriaceae Infections - genetics / Flavobacteriaceae Infections - microbiology / Gene expression / Genes / Genetic aspects / Genome, Bacterial - genetics / Genomes / Genomic analysis / Genomics / Genomics - methods / Glycoside hydrolase / Gram-negative bacteria / Gram-positive bacteria / Growth hormone / Humans / Hydrolases / Infections / Laboratories / Life Sciences / Medicine and Health Sciences / Microbial drug resistance / Nucleotides / Organic acids / Patients / Phylogeny / Physical Sciences / Polysaccharides / Prophages / Protein binding / Proteins / Resistance factors / Strains (organisms) / Studies / Sulfamethoxazole / Tetracyclines / Transcription (Genetics) / Transcription Factors - genetics / Trimethoprim / Viral infections / Virulence / Virulence (Microbiology) / Virulence factors / Virulence Factors - genetics
• ISBN: 0005324670000