Journal Article

Glial Membranes at the Node of Ranvier Prevent Neurite Outgrowth

Phillips, Greg R, Shan, Weisong, Mi, Sha, Florens, Laurence, Pedraza, Liliana, Colman, David R, Belkaid, Wiam, Roth, Alejandro D, Fex-Svenningsen, Asa, Huang, Jeffrey K, Yates, John R

2005

Overview

Nodes of Ranvier are regularly placed, nonmyelinated axon segments along myelinated nerves. Here we show that nodal membranes isolated from the central nervous system (CNS) of mammals restricted neurite outgrowth of cultured neurons. Proteomic analysis of these membranes revealed several inhibitors of neurite outgrowth, including the oligodendrocyte myelin glycoprotein (OMgp). In rat spinal cord, OMgp was not localized to compact myelin, as previously thought, but to oligodendroglia-like cells, whose processes converge to form a ring that completely encircles the nodes. In OMgp-null mice, CNS nodes were abnormally wide and collateral sprouting was observed. Nodal ensheathment in the CNS may stabilize the node and prevent axonal sprouting.

• Publisher: American Association for the Advancement of Science,The American Association for the Advancement of Science
• Subject: Accident Prevention / Analysis / Anatomy / Animals / Antigens - analysis / Axons / Axons - physiology / Axons - ultrastructure / Axons/physiology/ultrastructure / Biological and medical sciences / Brain / Cattle / Cell Surface Extensions - chemistry / Cell Surface Extensions - physiology / Cell Surface Extensions - ultrastructure / Cell Surface Extensions/chemistry/physiology/ultrastructure / Cells, Cultured / Central nervous system / Company growth / Fractions / Fundamental and applied biological sciences. Psychology / Ganglia, Spinal - physiology / Ganglia, Spinal - ultrastructure / Glia / glycoproteins / GPI-Linked Proteins / Growth / Humans / Inflammation / Injuries / Isolated neuron and nerve. Neuroglia / Mice / Myelin / Myelin Proteins / myelin sheath / Myelin Sheath - chemistry / Myelin-Associated Glycoprotein - analysis / Myelin-Oligodendrocyte Glycoprotein / nerve tissue / Nerves / Neurites / Neurites - physiology / Neurites - ultrastructure / Neurites/physiology/ultrastructure / Neuritis / Neuroglia / Neuroglia - chemistry / Neuroglia - physiology / Neuroglia - ultrastructure / Neuroglia/chemistry/physiology/ultrastructure / Neurology / Neurons / Neuroscience / oligodendroglia / Oligodendroglia - chemistry / Oligodendroglia - physiology / Oligodendroglia - ultrastructure / Oligodendroglia/chemistry/physiology/ultrastructure / Proteoglycans - analysis / Proteomics / Ranvier's Nodes - chemistry / Ranvier's Nodes - physiology / Ranvier's Nodes - ultrastructure / Ranvier's Nodes/chemistry/physiology/ultrastructure / Rats / Scientific Concepts / Spinal cord / Spinal Cord - cytology / Sprouting / Structure / Vertebrates: nervous system and sense organs / White matter