Journal Article

Rice stripe virus-derived siRNAs play different regulatory roles in rice and in the insect vector Laodelphax striatellus

Xu, Zhongtian, Zhang, Xiaoming, Lu, Lu, Liu, Renyi, Yang, Meiling, Liu, Qing, Cui, Feng, Zhao, Wan, Li, Qiong

2018

Overview

Background Most plant viruses depend on vector insects for transmission. Upon viral infection, virus-derived small interfering RNAs (vsiRNAs) can target both viral and host transcripts. Rice stripe virus (RSV) is a persistent-propagative virus transmitted by the small brown planthopper ( Laodelphax striatellus , Fallen) and can cause a severe disease on rice. Results To investigate how vsiRNAs regulate gene expressions in the host plant and the insect vector, we analyzed the expression profiles of small RNAs (sRNAs) and mRNAs in RSV-infected rice and RSV-infected planthopper. We obtained 88,247 vsiRNAs in rice that were predominantly derived from the terminal regions of the RSV RNA segments, and 351,655 vsiRNAs in planthopper that displayed relatively even distributions on RSV RNA segments. 38,112 and 80,698 unique vsiRNAs were found only in rice and planthopper, respectively, while 14,006 unique vsiRNAs were found in both of them. Compared to mock-inoculated rice, 273 genes were significantly down-regulated genes (DRGs) in RSV-infected rice, among which 192 (70.3%) were potential targets of vsiRNAs based on sequence complementarity. Gene ontology (GO) analysis revealed that these 192 DRGs were enriched in genes involved in kinase activity, carbohydrate binding and protein binding. Similarly, 265 DRGs were identified in RSV-infected planthoppers, among which 126 (47.5%) were potential targets of vsiRNAs. These planthopper target genes were enriched in genes that are involved in structural constituent of cuticle, serine-type endopeptidase activity, and oxidoreductase activity. Conclusions Taken together, our results reveal that infection by the same virus can generate distinct vsiRNAs in different hosts to potentially regulate different biological processes, thus reflecting distinct virus-host interactions.

• Publisher: BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
• Subject: Agriculture / Animals / Binding / Bioinformatics / Biological activity / Biomedical and Life Sciences / carbohydrate binding / Carbohydrates / Complementarity / Crop diseases / Cuticles / disease severity / Disease transmission / Diseases and pests / Endopeptidases / Environmental aspects / enzyme activity / Gene expression / gene expression regulation / Gene Expression Regulation, Plant / Gene Ontology / Genes / Genomes / Genomics / Hemiptera - genetics / Hemiptera - virology / Host plants / Host-Pathogen Interactions - genetics / host-pathogen relationships / Infections / Insect Proteins - genetics / insect vectors / Insect Vectors - genetics / Insect Vectors - virology / Insects / Kinases / Laodelphax striatellus / Life Sciences / messenger RNA / Ontology / Oryza - genetics / Oryza - virology / Oxidoreductase / Physiological aspects / Plant Diseases - virology / plant diseases and disorders / Plant Proteins - genetics / Plant Proteins - metabolism / Plant Sciences / Plant viruses / Plant-biotic interactions / Planthoppers / protein binding / Proteins / Research Article / Ribonucleic acid / Rice / Rice stripe tenuivirus / Rice stripe virus / RNA / RNA, Small Interfering - genetics / RNA, Viral / Segments / Serine / siRNA / Small brown planthopper / Small interfering RNA / Small RNA sequencing / Tenuivirus - genetics / Tenuivirus - pathogenicity / Transcriptome / Tree Biology / Viral genetics / Viral infections / Virus-derived small interfering RNAs / Viruses