Journal Article

Arterial Response to Shear Stress Critically Depends on Endothelial TRPV4 Expression

Hartmannsgruber, Veronika, Liedtke, Wolfgang, Grgic, Ivica, Hoyer, Joachim, Köhler, Ralf, Heyken, Willm-Thomas, Kaistha, Anuradha, Harteneck, Christian, Kacik, Michael

2007

Overview

In blood vessels, the endothelium is a crucial signal transduction interface in control of vascular tone and blood pressure to ensure energy and oxygen supply according to the organs' needs. In response to vasoactive factors and to shear stress elicited by blood flow, the endothelium secretes vasodilating or vasocontracting autacoids, which adjust the contractile state of the smooth muscle. In endothelial sensing of shear stress, the osmo- and mechanosensitive Ca(2+)-permeable TRPV4 channel has been proposed to be candidate mechanosensor. Using TRPV4(-/-) mice, we now investigated whether the absence of endothelial TRPV4 alters shear-stress-induced arterial vasodilation. In TRPV4(-/-) mice, loss of the TRPV4 protein was confirmed by Western blot, immunohistochemistry and by in situ-patch-clamp techniques in carotid artery endothelial cells (CAEC). Endothelium-dependent vasodilation was determined by pressure myography in carotid arteries (CA) from TRPV4(-/-) mice and wild-type littermates (WT). In WT CAEC, TRPV4 currents could be elicited by TRPV4 activators 4alpha-phorbol-12,13-didecanoate (4alphaPDD), arachidonic acid (AA), and by hypotonic cell swelling (HTS). In striking contrast, in TRPV4(-/-) mice, 4alphaPDD did not produce currents and currents elicited by AA and HTS were significantly reduced. 4alphaPDD caused a robust and endothelium-dependent vasodilation in WT mice, again conspicuously absent in TRPV4(-/-) mice. Shear stress-induced vasodilation could readily be evoked in WT, but was completely eliminated in TRPV4(-/-) mice. In addition, flow/reperfusion-induced vasodilation was significantly reduced in TRPV4(-/-) vs. WT mice. Vasodilation in response to acetylcholine, vasoconstriction in response to phenylephrine, and passive mechanical compliance did not differ between genotypes, greatly underscoring the specificity of the above trpv4-dependent phenotype for physiologically relevant shear stress. Genetically encoded loss-of-function of trpv4 results in a loss of shear stress-induced vasodilation, a response pattern critically dependent on endothelial TRPV4 expression. Thus, Ca(2+)-influx through endothelial TRPV4 channels is a molecular mechanism contributing significantly to endothelial mechanotransduction.

• Publisher: Public Library of Science,Public Library of Science (PLoS)
• Subject: Acetylcholine / Amino Acid Sequence / Animals / Arachidonic acid / Arteries / Biophysics/Cell Signaling and Trafficking Structures / Blood / Blood flow / Blood pressure / Blood vessels / Calcium influx / Calcium ions / Calcium permeability / Cardiovascular Disorders/Coronary Artery Disease / Cardiovascular Disorders/Hemodynamics / Cardiovascular Disorders/Hypertension / Cardiovascular Disorders/Vascular Biology / Carotid arteries / Carotid Arteries - metabolism / Carotid Arteries - physiology / Carotid artery / Cell size / Cellular signal transduction / Coding / Compliance / Endothelial cells / Endothelium / Endothelium, Vascular - metabolism / Endothelium, Vascular - physiology / Female / Genetic code / Genotypes / Immunohistochemistry / Male / Mechanical stimuli / Mechanotransduction / Mice / Mice, Knockout / Molecular Sequence Data / Muscle contraction / Nitric oxide / Organs / Oxygen / Patch-Clamp Techniques / Phenylephrine / Physiology/Cardiovascular Physiology and Circulation / Physiology/Cell Signaling / Physiology/Sensory Systems / Reperfusion / Rodents / Shear stress / Shear stresses / Smooth muscle / Stress, Mechanical / Transduction / TRPV Cation Channels - chemistry / TRPV Cation Channels - genetics / TRPV Cation Channels - physiology / Vasoactive agents / Vasoconstriction / Vasodilation / Veins & arteries